PXD065660

PXD065660 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis thaliana PSS1 TurboID N-teminal fusion
Description	The Arabidopsis PSS1 gene, which encodes a glycine-rich protein, plays a pivotal role in nonhost resistance to the soybean oomycete Phytophthora sojae and the fungal pathogen Fusarium virguliforme. To elucidate the molecular basis of PSS1-mediated immunity, we employed miniTurbo-based proximity labeling in stable transgenic Arabidopsis lines expressing miniTurbo fused to either wild-type PSS1 or its nonfunctional mutant variant, PSS1G119D. Seedlings were challenged with or without P. sojae, and biotinylated proteins were isolated and analyzed by mass spectrometry. The resulting PSS1 interactomes revealed several candidate proteins predominantly localized in the plastid and cytosol. Notably, proteins such as LIN2, ATPMEPCRA, and TGG1 were specifically enriched in wild-type PSS1 lines under P. sojae infection, suggesting their potential involvement in PSS1-mediated nonhost resistance. Our findings offer novel insights into immune protein networks and provide a foundational resource for dissecting nonhost immunity mechanisms in plants
HostingRepository	PRIDE
AnnounceDate	2026-06-11
AnnouncementXML	Submission_2026-06-11_10:30:49.911.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD065660
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Montes Serey
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; scientific name: Phytophthora sojae; NCBI TaxID: NEWT:67593;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-01 08:32:09	ID requested
⏵ 1	2026-06-11 10:30:50	announced

10.3389/fpls.2025.1701640;

Akintayo OF, Montes Serey C, Walley JW, Bhattacharyya MK, Proximity labelling suggests association of the nonhost receptor PSS1 protein with enzymes of multiple defense pathways in Arabidopsis. Front Plant Sci, 16():1701640(2025) [pubmed]

10.6019/PXD065660;

submitter keyword: nonhost resistance,Arabidopsis, protein interactome, proximity labeling, PSS1, miniTurbo, Phytophthora sojae

Madann K. Bhattacharyya
contact affiliation	Department of Agronomy Iowa State University
contact email	mbhattac@iastate.edu
lab head
Christian Montes Serey
contact affiliation	Iowa State University
contact email	chrisfm@iastate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD065660

PRIDE project URI

• PRIDE
  1. PXD065660
     1. Label: PRIDE project
     2. Name: Arabidopsis thaliana PSS1 TurboID N-teminal fusion