PXD065656

PXD065656 is an original dataset announced via ProteomeXchange.

Title	Direct in-bone protein digestion with subsequent LC separation and trapped ion mobility MS detection of released peptides as an effective tool for the proteomic characterization of bone tissues
Description	Common pathological changes in bone tissues like osteomas or exostoses remain not fully understood at the molecular level due to the difficulties in analyzing bone tissues in which they occur. Therefore, new rapid and powerful techniques are needed that could become routine tools for such analysis. The primary aim of this study was to evaluate whether direct in-bone tryptic protein digestion followed by LC separation and trapped ion mobility MS detection and identification of released peptides is able to identify sufficient numbers of proteins in above mentioned bone tissues. The second aim was to verify whether the mathematical analysis of the obtained MS data would have a potential to distinguish pathological and control healthy bone tissues. It turned out that this approach made possible to identify altogether 4810 proteins in samples of control healthy skull bone tissues, 6284 proteins in pathological skull bone tissues, and 3000 proteins in mandibular bone tissues. Mathematical analysis of obtained MS data enabled to discriminate control healthy and pathological skull bone tissues samples with accuracy of 87%.
HostingRepository	PRIDE
AnnounceDate	2025-09-18
AnnouncementXML	Submission_2025-09-18_11:09:59.644.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiri Santrucek
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-01 05:49:32	ID requested
⏵ 1	2025-09-18 11:10:00	announced

10.1002/JSSC.70277;

submitter keyword: skull base surgery, oral surgery, in-bone peptide separation, osteomas,exostosis

Radovan Hynek
contact affiliation	Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague
contact email	hynekr@vscht.cz
lab head
Jiri Santrucek
contact affiliation	University of Chemistry and Technology Prague
contact email	santrucj@vscht.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065656
     1. Label: PRIDE project
     2. Name: Direct in-bone protein digestion with subsequent LC separation and trapped ion mobility MS detection of released peptides as an effective tool for the proteomic characterization of bone tissues