PXD065655

PXD065655 is an original dataset announced via ProteomeXchange.

Title	Specialized shuttle proteins recognize and target type IX secretion effectors
Description	Members of the phylum Bacteroidota utilize the type IX secretion system (T9SS) to transport diverse substrates into the environment or onto their surface. T9SS substrates feature a Sec-dependent signal peptide for export to the periplasm and a conserved C-terminal domain (CTD), recognized by the T9SS, for translocation across the outer membrane. Following translocation, substrates engage with a shuttle protein, which ensures their final localization. Almost all CTDs are classified into Type A and Type B families. Well characterized Type A CTDs are all recognized by the PorV shuttle. However, recognition and transport of Type B CTDs remain less explored. Flavobacterium johnsoniae encodes 12 Type B substrates, most often genetically linked to genes encoding PorP/SprF-like shuttle proteins. Here, we demonstrate that two of these Type B substrates indeed rely on their cognate PorP/SprF specialized shuttle proteins for their secretion and identify the shuttle protein responsible for the secretion of three orphan Type B CTDs. Our findings also reveal that five conserved motifs within Type B CTDs are necessary for secretion but not sufficient for their specific recognition by cognate shuttle proteins. Our results further suggest that CTDs contain both a secretion signal, sufficient for recognition and secretion of substrates by the T9SS, and a targeting signal, which directs substrates to their final localization.
HostingRepository	PRIDE
AnnounceDate	2025-09-03
AnnouncementXML	Submission_2025-09-03_07:15:04.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AUDEBERT Stephane
SpeciesList	scientific name: Flavobacterium johnsoniae; NCBI TaxID: 986;
ModificationList	monohydroxylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-01 05:19:13	ID requested
⏵ 1	2025-09-03 07:15:04	announced

Dataset with its publication pending

submitter keyword: Type IX secretion, LC-MSMS, secretion signal, Bacteroidota, flavobacterium

Dr DOAN Thierry
contact affiliation	LISM – CNRS UMR7255 _ Aix Marseille University Team : Assembly of bacterial multi-protein complexes
contact email	tdoan@imm.cnrs.fr
lab head
AUDEBERT Stephane
contact affiliation	Marseille Proteomic, Centre de Recherche en Cancérologie de Marseille, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes, 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France
contact email	stephane.audebert@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD065655

PRIDE project URI

• PRIDE
  1. PXD065655
     1. Label: PRIDE project
     2. Name: Specialized shuttle proteins recognize and target type IX secretion effectors