PXD065630

PXD065630 is an original dataset announced via ProteomeXchange.

Title	Protein mass spectrometry raw data of M7A, M7GA, M7A-DC, and M7GA-DC for DAR calculation in study of MSLN-directed DARPin-drug conjugates
Description	Mesothelin (MSLN) is an attractive therapeutic target for precision cancer treatments. Here, we identify a MSLN-targeting DARPin, M7, which specifically binds to the protease-sensitive C-terminal region of MSLN. Furthermore, we develop two auristatin-based DARPin-drug conjugates (DARPin-DCs), M7A-DC and M7GA-DC. We show that M7A-DC and M7GA-DC are effectively internalized by MSLN-positive cells, leading to the release of MMAE that induces lethal effects on tumor cells and bystander killing against MSLN-negative cells. Both M7A-DC and M7GA-DC induce long-lasting tumor regression with favorable tolerability in mice.
HostingRepository	PRIDE
AnnounceDate	2025-09-05
AnnouncementXML	Submission_2025-09-05_00:32:36.758.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ying wang
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-30 18:46:18	ID requested
⏵ 1	2025-09-05 00:32:37	announced

10.1038/S41467-025-63269-6;

submitter keyword: LC/MS, protein-drug conjugates

Baorui Liu
contact affiliation	The Comprehensive Cancer Centre of Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, 321 Zhongshan Road, Nanjing, 210008, China.
contact email	baoruiliu@nju.edu.cn
lab head
ying wang
contact affiliation	Nanjing University
contact email	yvonnewang29@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD065630

PRIDE project URI

• PRIDE
  1. PXD065630
     1. Label: PRIDE project
     2. Name: Protein mass spectrometry raw data of M7A, M7GA, M7A-DC, and M7GA-DC for DAR calculation in study of MSLN-directed DARPin-drug conjugates