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PXD065541

PXD065541 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe first analysis of Spirometra mansoni excretory-secretory products by the 4D-DIA method
DescriptionBackground: To systematically analyze the components of excretory-secretory protein (ESP) of Spirometra mansoni. Methods: The adult and plerocercoid of S. mansoni were cultivated in vitro. Then, the differentially expressed proteins (DEPs) were enriched and protein components were examined using Data Independent Acquisition (DIA) mode. Furthermore, bioinformatics analysis was performed on DEPs. Results: A total of 944 proteins were identified, including 580 plerocercoid-specific proteins, while no specific proteins were found in adult. Quantitative analysis showed that 607 proteins exhibited significant differential expression, with 390 proteins upregulated in the plerocercoid and 217 upregulated in the adult. The Gene Ontology functional annotation showed that the upregulated proteins in the plerocercoid were significantly enriched in nitrogen compound metabolism, proteasome core complexes, and ion binding. Kyoto Encyclopedia of Genes and Genomes pathway enrichment revealed the DEPs were highly correlated with signal transduction, signal transportation, and catabolism pathways. Meanwhile, metabolic network analysis showed that key pathways included the pentose phosphate pathway and glycolysis/gluconeogenesis. The protein-protein interaction network further revealed that RL40 played a pivotal role in functional regulatory nodes. In addition, indirect ELISA showed that ES immunization induced a Th1/Th2 mixed immune response in mice, dominated by Th1. Cytokine detection further verified that ES had good immunogenicity, and could activate both humoral and cellular immune responses. Conclusions: This study reveals the differential expression profile of ESPs between the adult and plerocercoid of S. mansoni for the first time. Findings in this study may demonstrate that ESP in the plerocercoid can induce a Th1-biased Th1/Th2 mixed immune response, offering potential reference for the diagnosis and prevention of sparganosis.
HostingRepositoryiProX
AnnounceDate2025-06-26
AnnouncementXMLSubmission_2025-06-26_19:41:56.741.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFei Gao
SpeciesList scientific name: Spirometra erinaceieuropaei; NCBI TaxID: 99802;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Astral
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-06-26 19:41:43ID requested
12025-06-26 19:41:57announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Spirometra mansoni, plerocercoid, excretory secretory protein, comparative proteomics, immune response
Contact List
Xi Zhang
contact affiliationZhengzhou University
contact emailzhangxi@zzu.edu.cn
lab head
Fei Gao
contact affiliationZhengzhou University
contact emailgaofei199812@163.com
dataset submitter
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