PXD065525

PXD065525 is an original dataset announced via ProteomeXchange.

Title	PARP7 is a proteotoxic stress sensor which labels proteins for degradation
Description	ADP-ribosylation is a post-translational modification that plays a critical role in cellular stress responses. We have observed that during proteotoxic stress, cellular ADP-ribosylation increases, with ADP-ribosylated proteins accumulating in cytoplasmic foci containing ubiquitin and p62. During prolonged stress, these ADP-ribosylated proteins are transported to aggresomes and subsequently degraded via autophagy. In the absence of ubiquitination, ADP-ribosylated proteins become more prevalent and less soluble, indicating that ubiquitination is indispensable for this process. Upon inhibition of PARP7, accumulation of mono(ADP-ribosyl)ated proteins in response to proteotoxic stress is impeded. PARP7 turnover is very high under normal conditions, however, the protein stabilises following proteotoxic stress and thereby forms an ideal proteotoxic stress sensor. Finally, our findings imply that contrary to the current paradigm, perhaps not all ADP-ribosylation occurs on specific sites to regulate specific protein characteristics. Instead, it may be rather promiscuous to enable efficient protein degradation to prevent irreversible damage caused by defective proteins.
HostingRepository	PRIDE
AnnounceDate	2025-09-02
AnnouncementXML	Submission_2025-09-02_06:04:58.593.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mirna Barsoum
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-26 10:26:57	ID requested
⏵ 1	2025-09-02 06:04:58	announced

10.1038/S44318-025-00545-7;

submitter keyword: LC-MSMS,HUMAN

Karla Feijs
contact affiliation	Institute of Biochemistry and Molecular Biology, RWTH Aachen University
contact email	kfeijs@ukaachen.de
lab head
Mirna Barsoum
contact affiliation	Proteomics Facility, IZKF Uniklinik RWTH Aachen Pauwelsstrasse 30 52074 Aachen Germany
contact email	mbarsoum@ukaachen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065525
     1. Label: PRIDE project
     2. Name: PARP7 is a proteotoxic stress sensor which labels proteins for degradation