PXD065524

PXD065524 is an original dataset announced via ProteomeXchange.

Title	Convergent Activation of the Integrated Stress Response and ER–Mitochondria Uncoupling in VAPB-Associated ALS
Description	Human iPSC-derived motor neurons with either VAPB WT or VAPB P56S expressed under the control of a doxycycline inducible promoter were grown out to day 35 of motor neuron differentiation. A co-immunoprecipitation was then performed using the VAPB protein as bait to determine the interactome of VAPB and the effect of the P56S mutation upon it, specifically within motor neurons, the affected cell type in ALS.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:31:24.974.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD065524
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Belinda Willard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-26 09:54:51	ID requested
⏵ 1	2025-09-14 16:31:25	announced

10.6019/PXD065524;

10.1038/s44321-025-00279-3;

Landry C, Costanzo JP, Mitne-Neto M, Zatz M, Schaffer AE, Hatzoglou M, Muotri AR, Miranda HC, Convergent activation of the integrated stress response and ER-mitochondria uncoupling in VAPB-associated ALS. EMBO Mol Med, 17(9):2299-2331(2025) [pubmed]

submitter keyword: ALS Type VIII, Mitochondria, VAPB, ISR, ISRIB, ER-MAM,ALS, neurodegeneration

Helen Miranda
contact affiliation	Department of Genetics and Genome Sciences, CWRU School of Medicine
contact email	hcm34@case.edu
lab head
Belinda Willard
contact affiliation	Cleveland Clinic
contact email	willarb@ccf.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD065524

PRIDE project URI

• PRIDE
  1. PXD065524
     1. Label: PRIDE project
     2. Name: Convergent Activation of the Integrated Stress Response and ER–Mitochondria Uncoupling in VAPB-Associated ALS