PXD065495

PXD065495 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Integrin a5 Associated Complexes Dependent upon Integrin Activation State in Zebrafish
Description	Integrins are a major class of heterodimeric adhesion receptors composed of an a and b subunit that link the extracellular matrix (ECM) and the cytoskeleton across the cell membrane. When activated by either the intracellular (inside-out signaling) or extracellular (outside-in signaling) environment, integrins undergo a conformational change that increases the ligand binding affinity. As the primary receptor for ECM protein fibronectin, Integrin a5 plays a critical role in zebrafish somitogenesis. To better understand integrin activation in this context, we performed co-immunoprecipitation and Mass Spectrometry (MS) based proteomics using FLAG-tagged Integrin a5 alleles that alter it activation state: constitutive active mutant a5GAAKR and inactive ligand binding deficient mutant a5FYLDD, expressed in maternal zygotic a5 mutant (MZa5-/-) embryos. Our data provide an overview of Integrin associated proteins according to the activation state of the Integrin. This submission contains the raw data files corresponding to PXD024942.
HostingRepository	PRIDE
AnnounceDate	2025-10-13
AnnouncementXML	Submission_2025-10-12_16:34:52.001.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guangyu SUN
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: NEWT:7955;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-26 01:52:16	ID requested
⏵ 1	2025-10-12 16:34:52	announced

Sun G, Holley SA, 5 with actin in zebrafish somites. Mol Cell Proteomics, ():101087(2025) [pubmed]

10.1016/j.mcpro.2025.101087;

submitter keyword: integrin activation, LC-MSMS, 12 somite stage,Integrin a5, zebrafish, cell adhesion, extracellular matrix

Scott A. Holley
contact affiliation	Department of Molecular, Cellular and Developmental Biology. Yale University, 260 Whitney Ave, New Haven, CT. 06520, USA
contact email	scott.holley@yale.edu
lab head
Guangyu SUN
contact affiliation	Xiamen Cardiovascular Hospital of Xiamen University, School of Medicine, Xiamen University
contact email	sunguangyu@xmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD065495

PRIDE project URI

• PRIDE
  1. PXD065495
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Integrin a5 Associated Complexes Dependent upon Integrin Activation State in Zebrafish