PXD065491

PXD065491 is an original dataset announced via ProteomeXchange.

Title	Gamma secretase complex and DOCK2 affinity interactome
Description	γ-Secretase is a transmembrane protease complex responsible for processing multiple type I transmembrane proteins, including the amyloid precursor protein (APP) and NOTCH. Using a proteomics-based screening approach, we found that dedicator of cytokinesis protein 2 (DOCK2) interacts with the γ-secretase complex component Nicastrin (NCSTN), regulating NCSTN N45-mannosylation and the effects of γ-secretase on NOTCH receptors. Both genetically depleting DOCK2 and pharmacologically targeting NCSTN mannosylation via Kifunensine ameliorate Notch-dependent leukemia progression in vivo. Together, these results reveal a mechanism by which γ-secretase specifically recognizes its substrate and provide a direction for treating Notch-related diseases.
HostingRepository	iProX
AnnounceDate	2025-06-26
AnnouncementXML	Submission_2025-06-25_23:43:27.709.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhuo Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-25 23:43:11	ID requested
⏵ 1	2025-06-25 23:43:28	announced

Dataset with its publication pending

submitter keyword: Gamma secretase complex, NCSTN, Glycosylation Description

Xu Li
contact affiliation	Westlake University
contact email	lixu@westlake.edu.cn
lab head
Zhuo Zhang
contact affiliation	Dalian University of Technology
contact email	1078152767@qq.com
dataset submitter

iProX dataset URI