⮝ Full datasets listing
PXD065473
PXD065473 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteome-wide analysis and surface protein isolation for secretome characterization reveal insights into the biology of the leaf-cutter ant Acromyrmex echinatior |
| Description | The leaf-cutter ant Acromyrmex echinatior secretes a protein layer that covers their exoskeleton and physically interacts with biotic and abiotic factors, including their symbiotic bacteria Pseudonocardia. In this study, in order to characterize both the global proteome and the externally-secreted cuticular protein layer of A. echinatior, we utilize a novel, dual-layered proteomic approach. Using diaPASEF, we quantified 4,428 proteins across four early adult stages, uncovering distinct age-dependent protein clusters enriched in muscle development, lipid metabolism, and immune-related responses. We then developed a novel acid-based extraction method to isolate the externally-secreted protein layer, identifying 323 secreted proteins via ddaPASEF acquisition, many of which were temporally upregulated and associated with various functions such as environmental stress response, microbial defense, and cuticle structural maturation. Notably, tropomyosin-family proteins were both highly enriched in the external-secretome and exhibited significant changes across the early adult timepoints, potentially linking these ion-binding molecules to the metal-enrichment processes that takes place during this crucial stage. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-06 |
| AnnouncementXML | Submission_2025-12-06_13:23:18.641.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Penghsuan Huang |
| SpeciesList | scientific name: Acromyrmex echinatior; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF fleX MALDI-2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-06-25 20:07:00 | ID requested | |
| ⏵ 1 | 2025-12-06 13:23:19 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Leaf-cutter ant, secretome, biomineralization, DatasetType:Proteomics |
Contact List
| Lingjun Li | |
|---|---|
| contact affiliation | University of Wisconsin-Madison |
| contact email | lingjun.li@wisc.edu |
| lab head | |
| Penghsuan Huang | |
| contact affiliation | UW-Madison |
| contact email | penghsuan.huang@wisc.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098331/ |
