PXD065462

PXD065462 is an original dataset announced via ProteomeXchange.

Title	Targeting the translation machinery: The RNase Y specificity factor Y-complex coordinates ribosome degradation
Description	Limiting the synthesis and activity of ribosomes is crucial for responding to various stresses, including nutrient starvation or proteotoxic stress. In Bacillus subtilis, this is achieved through the coordinated action of the alarmones (p)ppGpp and the transcription factor Spx. In this study, we performed a genetic screening for new factors involved in the heat shock response of B. subtilis. We discovered that the RNase Y specificity factor Y-complex is important for the heat shock response and the transition to the stationary growth phase. The Y-complex is required for processing different RNAs, including mRNA maturation. We further demonstrate that the Y-complex and RNase Y initiate rRNA degradation in mature ribosomes, lowering their abundance. We propose the Y-complex as a system by which B. subtilis regulates protein synthesis and resource allocation at the entry to stationary phase.
HostingRepository	PRIDE
AnnounceDate	2026-04-09
AnnouncementXML	Submission_2026-04-09_07:18:05.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathirvel Alagesan
SpeciesList	scientific name: Bacillus subtilis; NCBI TaxID: NEWT:1423;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-25 13:50:29	ID requested
⏵ 1	2026-04-09 07:18:06	announced

Dataset with its publication pending

submitter keyword: RNase Y, ribosome, Bacillus subtilis

Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens Charitéplatz 1,D-10117 Berlin, Germany
contact email	alagesan@mpusp.mpg.de
lab head
Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	alagesan@mpusp.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065462
     1. Label: PRIDE project
     2. Name: Targeting the translation machinery: The RNase Y specificity factor Y-complex coordinates ribosome degradation