PXD065453 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | LC-ESI-MS/MS on a purified recombinant protein sample to confirm its amino acid sequence and a certain cleavage site |
| Description | The study entails the design, production and in vitro testing of a novel recombinant anti-B7-H3 affibody coupled with a small cytotoxic protein, with considerable potential in Acute Myeloid Leukemia (AML) immunotherapy. The protein construct was expressed in E. coli. After affinity purification and validation of its sequence through LC-MS/MS, the obtained protein’s efficacy was tested on AML representative B7-H3-positive THP-1 cells and B7-H3-negative RAJI cells. The IC50 of the cytotoxic protein was determined through MTS assay and its apoptotic and necrotic activities on the two cell lines were evaluated through flow cytometry and Western blot. Taken together, the results show that the cytotoxic anti-B7-H3 affibody we produced possesses highly necrotic and apoptotic targeted effects that are specific to only B7-H3-expressing THP-1 cells and not the RAJI control line. Moreover, these observed effects are significantly more potent than the effects of the cytotoxin alone, as they are described in previous studies. In conclusion, we designed, produced and evaluated a novel anti-B7-H3 cytotoxic affibody that from these preliminary in vitro data, shows high potential for translation to the immunotherapy of AML. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-10-24 |
| AnnouncementXML | Submission_2025-10-24_09:32:10.552.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Cristian Munteanu |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-25 10:14:49 | ID requested | |
| ⏵ 1 | 2025-10-24 09:32:11 | announced | |
Publication List
| Vasilescu AM, Vasilescu AG, Sima LE, Munteanu CVA, Baran N, Szedlacsek Ș E, A novel cytotoxic anti-B7-H3 affibody with therapeutic potential in acute myeloid leukemia. Front Pharmacol, 16():1684226(2025) [pubmed] |
| 10.3389/fphar.2025.1684226; |
Keyword List
| ProteomeXchange project tag: Cancer (B/D-HPP), Antibodies (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
| submitter keyword: Affibody, B7-H3, Recombinant, Cytotoxin,LC-ESI-MS/MS, AML |
Contact List
| Stefan Szedlacsek |
|---|
| contact affiliation | Head of Enzymology Department, Institute of Biochemistry of the Romanian Academy (IBRA) |
| contact email | stefan@biochim.ro |
| lab head | |
| Cristian Munteanu |
|---|
| contact affiliation | Institute of Biochemistry of the Romanian Academy (IBRA) |
| contact email | cristian.v.a.munteanu@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD065453 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065453
- Label: PRIDE project
- Name: LC-ESI-MS/MS on a purified recombinant protein sample to confirm its amino acid sequence and a certain cleavage site
