PXD065448 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | U2AF2 controls alternative splicing in speckles proximal regions in an RS domain-dependent manner |
| Description | Splicing factor U2AF2 is known to play a pivotal role for 3' splice site recognition at an early step of spliceosome assembly. Here using proximity labeling and biochemical confirmations, we extend the repertoire of putative functional partners of U2AF2 mainly for splicing, chromatin modification, transcription, 3' end processing and RNA methylation. Removal of its RS domain alters numerous of these interactions, reduces its localisation in speckles and impacts splicing genome wide in a manner that depends both on splicing signals and on introns length. Indeed cassette exon flanked by short introns in genes or transcripts located closed to speckles are the most affected by U2AF2 knockdown or RS domain removal. Interestingly, our bioinformatic analyses further reveal that alternative splicing in general mainly occurs near speckles. Finally, we show that multiple phosphorylation sites within the U2AF2 RS domain are required for normal splicing, suggesting that its RS domain mediates U2AF2 regulations. Altogether our data indicate that U2AF2 is in limiting amount to ensure cassette exon inclusion near speckles and give a novel insight on the mechanism of alternative splicing. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-09 |
| AnnouncementXML | Submission_2026-03-08_17:44:25.104.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD065448 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Laïla sago |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | biotinylated residue |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-25 08:38:16 | ID requested | |
| ⏵ 1 | 2026-03-08 17:44:26 | announced | |
Publication List
| 10.6019/PXD065448; |
| 10.1093/nar/gkag143; |
| Pankivskyi S, Kobayashi A, Salone JM, Gargoly K, Pastr, é D, Maucuer A, U2AF2 controls alternative splicing in speckle-proximal regions in an RS domain-dependent manner. Nucleic Acids Res, 54(5):(2026) [pubmed] |
Keyword List
| submitter keyword: U2AF2/ RS domain/ alternative splicing/ nuclear sepckles/ phosphorylation |
Contact List
| Maucuer Alexandre |
|---|
| contact affiliation | INSERM, laboratory: INSERM UMR1204, Structure-Activité des Biomolécules Normales et Pathologiques, Université d'Evry/Paris-Saclay France |
| contact email | alexandre.maucuer@inserm.fr |
| lab head | |
| Laïla sago |
|---|
| contact affiliation | I2BC, CNRS |
| contact email | laila.sago@i2bc.paris-saclay.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD065448 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065448
- Label: PRIDE project
- Name: U2AF2 controls alternative splicing in speckles proximal regions in an RS domain-dependent manner
