PXD065442

PXD065442 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Genome Mining of Monoterpenes from Ascidian-Derived fungus Diaporthe sp. SYSU-MS4722
Description	Marine-derived fungi represent anunderexplored sourceofmonoterpenes, withonlysporadicreportsover thepast twodecades,makingtheir discovery particularlychallenging.Whole-genomesequencing of Diaporthesp. SYSU MS4722,anascidian-derivedfungusisolatedfromStyelaplicata, identified130 biosyntheticgeneclusters(BGCs),underscoringitsvastbiosyntheticpotential. However, previous studies demonstrated that Diaporthe sp. SYSU-MS4722 predominantlyproduces xanthone phomoxanthone A understandardlaboratory conditions, suggestingthatmanyBGCs remaincryptic.Notably, 16 of these BGCs encode terpene synthase genes, indicatingthepotentialformonoterpene biosynthesis. To activate these silent BGCs and discover monoterpenes, we deleted sthe polyketide synthase (PKS) gene phoE, responsible for phomoxanthoneAbiosynthesis, generating themutant strainDiaporthe sp. SYSU-MS4722 phoE, fromwhich threenewmonoterpenes, diaporterpenes D-F (1-3), three knownmonoterpenes (4-6), and twonewpolypropionate derivatives, diaporpolypropionateA (7) anddiaporpolypropionateB (8),were isolated.Compounds1,4, 7, and8wereevaluatedfor theircytotoxicityand anti-inflammatory effects inhumannon-small cell lungcancer A549cells. Compound 1 exhibitedcytotoxic activitywith an IC50 valueof 89.33 M. Compounds4and7demonstratedanti-inflammatoryactivity,asmeasuredby anELISAassayassessingthe inhibitionof IL-6secretion,withEC50 values of 41.85 Mand70.80 M,respectively.
HostingRepository	PRIDE
AnnounceDate	2025-07-04
AnnouncementXML	Submission_2025-07-04_05:48:37.016.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu hang Wen
SpeciesList	scientific name: Diaporthe sp. T1; NCBI TaxID: 1857017;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-25 07:12:52	ID requested
⏵ 1	2025-07-04 05:48:37	announced

Publication List

10.3389/FMICB.2025.1593027;

10.3389/FMICB.2025.1593027;

Keyword List

submitter keyword: genome mining Q10 , phomoxanthone A, monoterpenes, antiinflammatoryactivity, polypropionate derivatives

submitter keyword: genome mining Q10 , phomoxanthone A, monoterpenes, antiinflammatoryactivity, polypropionate derivatives

Contact List

Yuhang Wen
contact affiliation	Zunyi medical University
contact email	1761560326@qq.com
lab head
Yu hang Wen
contact affiliation	18519891289
contact email	1761560326@qq.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD065442
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD065442

PRIDE project URI

Repository Record List

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