PXD065439

PXD065439 is an original dataset announced via ProteomeXchange.

Title	Passive transfer of IgG immune complex from ME/CFS patients alters mitochondrial architecture and function
Description	Autoimmunity is an established clinical feature in both Myalgic encephalomyelitis / chronic fatigue syndrome (ME/CFS) and Post-Acute Sequelae of COVID (PASC). Passive transfer of immunoglobulins from patients' sera into mice induces some clinical features of PASC. IgG-induced transfer of disease phenotypes has long been appreciated, yet the exact mechanism of disease development remains largely elusive. Here, we show that IgG isolated from ME/CFS patients selectively induces mitochondrial fragmentation in primary human endothelial cells and alters mitochondrial energetics. Blocking IgG entry into cells using an Fc blocker inhibits the mitochondrial fragmentation phenotype. Both the digested Fab and Fc regions of IgG separately entered the endothelial cells. However, only the Fab fragment alone was able to alter the mitochondrial energetics, similar to native IgG. Proteomics analysis of IgG-bound immune complex revealed significant and specific changes within the immune complex of ME/CFS and PASC patients compared to healthy controls and other disease controls. We demonstrate that IgGs from ME/CFS patients carry a chronic protective response signal that promotes mitochondrial adaptation to stress through mitochondrial fragmentation, without altering mitochondrial ATP generation capacity in endothelial cells. Our results suggest that chronic and pathogenic IgG-induced mitophagy and metabolic alterations to stress-dependent ATP production may lead to cellular saturation, ultimately affecting mitochondrial health. These results provide an opportunity to develop alternative therapeutic intervention strategies.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_20:56:56.881.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bhupesh Kumar Prusty
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-25 06:50:25	ID requested
⏵ 1	2026-02-22 20:56:57	announced

10.1016/j.bbih.2026.101187;

Liu Z, Hollmann C, Kalanidhi S, Lamer S, Schlosser A, Basens EE, Nikolayshvili G, Sokolovska L, Riemekasten G, Rust R, Bellmann-Strobl J, Paul F, Naviaux RK, Nora-Krukle Z, Sotzny F, Scheibenbogen C, Prusty BK, Immunoglobulin G complexes from post-infectious ME/CFS, including post-COVID ME/CFS disrupt cellular energetics and alter inflammatory marker secretion. Brain Behav Immun Health, 52():101187(2026) [pubmed]

ProteomeXchange project tag: Antibodies (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Serum IgG Proteomics, ME/CFS, MS

Bhupesh Kumar Prusty
contact affiliation	Institute for Microbiology and Virology, Riga Stradins University, Riga, Latvia
contact email	bhupesh.prusty@rsu.lv
lab head
Bhupesh Kumar Prusty
contact affiliation	Riga Stradins University
contact email	bhupesh.prusty@rsu.lv
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD065439

PRIDE project URI

• PRIDE
  1. PXD065439
     1. Label: PRIDE project
     2. Name: Passive transfer of IgG immune complex from ME/CFS patients alters mitochondrial architecture and function