PXD065436

PXD065436 is an original dataset announced via ProteomeXchange.

Title	EDC exposure during SC-islet differentiation
Description	We aimed to asses how EDC exposure in combination with a genetic predisposition for diabetes could impact pancreatic endocrine cell development. For this, we used a protocol to generate Stem-cell islets (SC-islets) from induced pluripotent stem cells (iPSCs) following a 7 satege protocol. For 2 weeks after pancreatic endocrine progenitor stage, cells were treated with a EDC mix of 1 nm of Bisphenol A, bisphenol S, and trans-nonachlor, or the vehicle DMSO. After those 2 weeks, the cells got a 1 week recovery period before collection. In the present study we used both iPSCs carrying the P291fsinC mutation in the HNF1A gene, and and isogenic non carrier control.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:09:43.817.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Aga Legøy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-25 05:27:11	ID requested
⏵ 1	2026-02-08 16:09:44	announced

10.1093/toxsci/kfaf163;

Gudmestad JH, Unger L, Paulo JA, Ghila L, Leg, ø, y TA, Endocrine-disrupting chemical exposure during differentiation alters the proliferation-maturation balance in stem-cell islets. Toxicol Sci, 209(1):(2026) [pubmed]

submitter keyword: SC-islets, iPSCs, EDC

Simona Chera
contact affiliation	Department of Clinical Science, Faculty of Medicine, University of Bergen, Norway
contact email	simona.chera@uib.no
lab head
Thomas Aga Legøy
contact affiliation	University of Bergen
contact email	tle088@uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD065436

PRIDE project URI

• PRIDE
  1. PXD065436
     1. Label: PRIDE project
     2. Name: EDC exposure during SC-islet differentiation