PXD065402

PXD065402 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of Pleurotus ostreatus mycelia grown in submerged fermentation under different light wavelength
Description	Light is a key environmental factor in the growth, development, and metabolism of the edιble mushroom Pleurotus ostreatus. In this study, the effect of white, blue, green, yellow, red light, and darkness on the global protein expression profile of P. ostreatus LGAM 1123 grown in submerged cultivation was investigated. No inhibition of the growth of the fungus, in all the light conditions tested, compared to the dark, was observed. However, the mycelial protein content was reduced by 10% in blue and white light. From the proteomic analysis, a different proteome was expressed for each light wavelength, with red and blue light presenting the most distinctive proteome profiles. Blue light induces pathways such as the citrate cycle (TCA cycle), glycolysis/gluconeogenesis and biosynthesis of amino acids, while red light induces mRNA-related pathways. GC-MS analysis of biomass revealed differences in the produced amino acids, sugars, and lipids. The distinct regulation of proteins and bioactive compounds under different light wavelengths growth indicate that using a specific light wavelength, the metabolism of P. ostreatus could turn into particular biochemical pathways. These strategies could be useful for the food industry because specific nutrients could be increased in the fermentation of edible fungi without the need for genetic engineering of the strain.
HostingRepository	PRIDE
AnnounceDate	2025-10-15
AnnouncementXML	Submission_2025-10-15_09:54:49.841.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Pleurotus ostreatus; NCBI TaxID: NEWT:5322;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-24 07:55:14	ID requested
⏵ 1	2025-10-15 09:54:50	announced

10.3390/HORTICULTURAE11101234;

submitter keyword: proteomics, GC-MS,Light, photoreceptors, fungi, LC-MS?MS, biomolecules

Petros Katapodis
contact affiliation	Biotechnology Laboratory, Department of Biological Applications and Technologies, Universityof Ioannina, 45110 Ioannina, Greece
contact email	pkatapo@uoi.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065402
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of Pleurotus ostreatus mycelia grown in submerged fermentation under different light wavelength