PXD065392

PXD065392 is an original dataset announced via ProteomeXchange.

Title	A large-scale map of transient and constitutive interactors of human TOM complex enabled by crosslinking
Description	The translocase of the outer mitochondrial membrane (TOM) is the major entry gate for the over 1,000 proteins that are continuously being imported into the organelle. While the core TOM complex is evolutionarily preserved between yeast and human, the human mitochondrial proteome is significantly larger and thus the client range of the TOM complex is more extensive. An understanding of the multi-layered functions and regulations of the human TOM complex requires a global unbiased approach. We here describe the interactome of TOM complex affinity-purified from human mitochondria with high confidence. We find a large overlap with known homologous interactors from yeast. In addition, we extend the interactome to include transient and labile interactors, stabilized by a membrane-permeable crosslinker. Upon crosslinking prior to affinity purification, we significantly boost the fold change of the receptor and co-chaperone TOMM70, as well as of several other known quality control factors at the TOM complex, including the recently described ATAD1. Importantly, the dataset further harbors a number of yet un-described protein-protein interaction candidates that are boosted by the crosslinking. To validate these novel interaction candidates, we localized 24 unique inter-crosslinks to TOM core members.
HostingRepository	PRIDE
AnnounceDate	2026-03-17
AnnouncementXML	Submission_2026-03-17_09:42:21.526.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tereza Kadavá
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-24 05:46:34	ID requested
⏵ 1	2026-03-17 09:42:22	announced

Dataset with its publication pending

submitter keyword: Bottom-up MS, Cross-linking MS, Affinity purification MS,Human mitochondria, TOM complex

Albert JR Heck
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, Utrecht 3584 CH, the Netherlands and Netherlands Proteomics Center, Padualaan 8, Utrecht 3584 CH, the Netherlands
contact email	a.j.r.heck@uu.nl
lab head
Tereza Kadavá
contact affiliation	Utrecht University, Biomolecular Mass Spectrometry and Proteomics
contact email	t.kadava@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD065392

PRIDE project URI

• PRIDE
  1. PXD065392
     1. Label: PRIDE project
     2. Name: A large-scale map of transient and constitutive interactors of human TOM complex enabled by crosslinking