PXD065373

PXD065373 is an original dataset announced via ProteomeXchange.

Title	Clinically Relevant Aβ42 Stereochemistry-Driven Proteome Perturbation, Aggregation Crosstalk and Cytotoxicity Inhibition: Cytotoxicity
Description	The stereochemical modification of Aβ42 discovered in the clinical samples of Alzheimer's disease (AD) patients has attracted increasing attention. However, most existing studies have focused on the characteristic analysis of a single isomer, ignoring the complex interactions of the coexistence of multiple stereochemical isomers in the physiological environment, in particular, the crosstalk effects between D-Asp and/or D-Ser modifications and natural L-type Aβ42. This study found through systematic analysis that the coexistence of L-type and stereoisomerized Aβ42 monomers showed antagonistic effects that mutually inhibited fibril formation and promoted the generation of unstable fibrils through the seed effect. This crosstalk effect shows obvious neuroprotective effects at the cellular level, and its molecular mechanism involves the adaptive regulation of ribosome function and mitochondrial pathways. This study reveals for the first time the crosstalk effect of stereoisomerized Aβ42, breaking through the limitations of traditional single isomer research. This discovery provides a new perspective for understanding the pathogenesis of AD and developing new treatment strategies based on stereochemical regulation.
HostingRepository	PRIDE
AnnounceDate	2026-02-20
AnnouncementXML	Submission_2026-02-20_03:49:51.311.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gongyu Li
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-23 21:31:02	ID requested
⏵ 1	2026-02-20 03:49:51	announced

Dataset with its publication pending

submitter keyword: Aβ42

Alzheimer's disease

stereoisomeric modification

crosstalk effect

amyloid protein aggregation

neurotoxicity

ABPP;

Gongyu Li
contact affiliation	Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Science, Frontiers Science Center for New Organic Matter, College of Chemistry, Nankai University, Tianjin 300071, China
contact email	ligongyu@nankai.edu.cn
lab head
Gongyu Li
contact affiliation	Nankai University
contact email	ligongyu@nankai.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD065373

PRIDE project URI

• PRIDE
  1. PXD065373
     1. Label: PRIDE project
     2. Name: Clinically Relevant Aβ42 Stereochemistry-Driven Proteome Perturbation, Aggregation Crosstalk and Cytotoxicity Inhibition: Cytotoxicity