PXD065357

PXD065357 is an original dataset announced via ProteomeXchange.

Title	19S proteasome loss regulates mitotic spindle assembly through a ubiquitin-independent degradation mechanism
Description	During regulated protein degradation, the 26S proteasome recognizes ubiquitinated substrates through its 19S particle and then degrades them in its 20S enzymatic core. Despite this close interdependency between proteasome subunits, we demonstrate that knockouts from different proteasome subcomplexes result in distinct cellular phenotypes. In particular, depletion of 19S PSMD lid proteins, but not that of other proteasome subunits, prevents bipolar spindle assembly during mitosis. Despite decreased ubiquitin-mediated protein degradation in PSMD knockouts, we find that the monopolar spindle phenotype is instead caused by the aberrant degradation of the kinesin motor protein KIF11. We show that KIF11 degradation occurs through the 20S proteasome in a ubiquitin-independent manner upon loss of 19S proteins and that the resulting alterations in spindle forces lead to the unique monopolar phenotype. Thus, the presence of the 19S particle ensures proper spindle formation by restraining ubiquitin-independent degradation.
HostingRepository	PRIDE
AnnounceDate	2025-06-24
AnnouncementXML	Submission_2025-06-24_07:55:22.157.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD065357
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Océane Marescal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-23 16:54:04	ID requested
⏵ 1	2025-06-24 07:55:22	announced

10.6019/PXD065357;

submitter keyword: proteasome, mitosis, spindle, degradation, kinesin, ubiquitin

Iain Cheeseman
contact affiliation	Massachusetts Institute of Technology
contact email	icheese@wi.mit.edu
lab head
Océane Marescal
contact affiliation	Masschusetts Institute of Technology
contact email	oceanema@wi.mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065357
     1. Label: PRIDE project
     2. Name: 19S proteasome loss regulates mitotic spindle assembly through a ubiquitin-independent degradation mechanism