PXD065348

PXD065348 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Polyamines sustain epithelial regeneration in aged intestines by modulating protein homeostasis
Description	Aging hampers the regenerative potential of intestinal epithelium across species including humans, yet the underlying causes remain elusive. Here, using proteomic and metabolomic profiling of intestinal tissues together with functional assays, we characterized the temporal dynamics of regeneration following injury induced by 5-fluorouracil, a commonly used chemotherapeutic agent. Comparison of regeneration dynamics in mice of different ages revealed the emergence of proteostasis stress and increased levels of polyamines following injury exclusively in old epithelia. We show that delayed regeneration is an intrinsic feature of aged epithelial cells that display reduced protein synthesis and accumulation of ubiquitylated proteins. Inhibition of the polyamine pathway in vivo further delays regeneration in old mice, while its activation by dietary intervention or supplementation of polyamines is sufficient to enhance the regenerative capacity of aged intestines. Our findings highlight promising epithelial targets for interventions aimed at tackling the decline in tissue repair mechanisms associated with aging.
HostingRepository	MassIVE
AnnounceDate	2025-09-17
AnnouncementXML	Submission_2025-09-17_06:29:18.965.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leibniz FLI Proteomics
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-23 09:33:48	ID requested
⏵ 1	2025-09-17 06:29:19	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: polyamines, regeneration, intestines, protein homeostasis, DatasetType:Proteomics

submitter keyword: polyamines, regeneration, intestines, protein homeostasis, DatasetType:Proteomics

Contact List

Alessandro Ori
contact affiliation	Leibniz Institute on Aging Fritz Lipmann Institute (FLI)
contact email	alessandro.ori@leibniz-fli.de
lab head
Leibniz FLI Proteomics
contact affiliation	Leibniz FLI
contact email	proteomics@leibniz-fli.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098281/