PXD065338

PXD065338 is an original dataset announced via ProteomeXchange.

Title	The Escherichia coli RelB antitoxin C-terminus is essential for RelE toxin suppression and transcriptional repression.
Description	Bacterial type II toxin-antitoxin (TA) systems exhibit high specificity within each pair to ensure precise recognition of the toxin by its cognate antitoxin to inhibit toxicity of the free toxin. Despite high structural similarity among some TAs, crosstalk between noncognate TA pairs is rare. To determine how the E. coli RelB antitoxin suppresses its cognate RelE toxin, we engineered C-terminal truncations of RelB and tested their functional effects on toxin suppression in E. coli. We find that removal of the long Cterminal a3 and connecting loop 4 (L4) of RelB prevents RelE suppression. Quantitative binding assays of RelE and RelB variants support a reduced affinity upon removal of the RelB C-terminus. Disrupting these interactions between RelB and RelE also led to a significant decrease in transcriptional repression at the relO operator, underscoring the requirement for RelE binding to RelB for optimal repression at DNA repressor elements. Comparison to other structurally homologous TA systems, such as E. coli DinJ-YafQ, reveals key differences in the molecular mechanisms of both toxin suppression and DNA repressor activity highlighting the diversity in TA regulation and function.
HostingRepository	PRIDE
AnnounceDate	2025-07-06
AnnouncementXML	Submission_2025-07-06_15:02:34.344.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christine Dunham
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-23 07:23:38	ID requested
⏵ 1	2025-07-06 15:02:34	announced

10.1016/J.JBC.2025.110389.;

submitter keyword: relB,E coli, antitoxin

Dunham, Christine
contact affiliation	Director, Biochemistry, Cell and Developmental Biology Graduate Program Emory University, 450 Atwood Atlanta, Georgia 30322
contact email	cmdunha@emory.edu
lab head
Christine Dunham
contact affiliation	Emory University
contact email	labdunham223@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065338
     1. Label: PRIDE project
     2. Name: The Escherichia coli RelB antitoxin C-terminus is essential for RelE toxin suppression and transcriptional repression.