PXD065314

PXD065314 is an original dataset announced via ProteomeXchange.

Title	MAP2Ks can activate MAP kinases using ADP in vitro
Description	Kinases are a diverse group of enzymes that use ATP to phosphorylate a variety of substrates. Protein kinases evolved in eukaryotes as important mediators of cell signalling that target specific amino acid side chains to modulate downstream protein function. Among them, the MAPKs (mitogen-activated protein kinases) are a family of intracellular protein kinases that form signalling cascades responding to a number of stimuli, that control fundamental mechanisms such as proliferation, differentiation, inflammation and cell death. Signals propagate through consecutive kinases which eventually phosphorylate and activate a MAPK. Here, we show that the dual specificity threonine/tyrosine MAP kinase kinases (MAP2Ks or MEKs) are able to phosphorylate and activate their substrate MAPKs using ADP as well as ATP in vitro. As the pathways are involved in the stress response, we speculate that it would represent an advantage to be able to maintain signalling under conditions such as hypoxia, that occur under a number of cell stresses, including cancer and atherosclerosis, where the available pool of ATP could be depleted.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:06:14.143.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-23 01:44:59	ID requested
⏵ 1	2025-09-28 16:06:14	announced

10.1016/j.jbc.2025.110578;

Juyoux P, von Velsen J, Pellegrini E, Bowler MW, Dual-specificity mitogen-activated protein kinase kinases can use ADP to phosphorylate MAP kinases invitro. J Biol Chem, 301(9):110578(2025) [pubmed]

submitter keyword: kinase, ADP, phosphorylation, MAPK

Matthew W. Bowler
contact affiliation	European Molecular Biology Laboratory, 71 avenue des Martyrs, CS 90181 F-38042 Grenoble, France
contact email	mbowler@embl.fr
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065314
     1. Label: PRIDE project
     2. Name: MAP2Ks can activate MAP kinases using ADP in vitro