PXD065303

PXD065303 is an original dataset announced via ProteomeXchange.

Title	Hypoxia Induces HIF-1α Activation in Tendon Stem Cells to Enhance Extracellular Vesicle-mediated Tendon Repair
Description	Tendon injuries are challenging to treat due to the limited blood supply and low cell density in tendon tissue. Tendon stem cells (TSCs) typically reside in a hypoxic environment (1%-5% oxygen), which may be crucial for their physiological function. In this study, we demonstrated that under hypoxic conditions, TSCs release small extracellular vesicles (sEVs) enriched with functional molecules (mainly non-coding RNAs and proteins). These sEVs significantly reduced fibrosis and inflammation in a rat model of Achilles tendon injury, while enhancing the expression of tendon-related factors, thus promoting tendon healing and improving tissue structure and function. In vitro experiments revealed that hypoxia-induced TSC-derived sEVs not only promoted TSC differentiation but also inhibited the conversion of tenocytes to myofibroblasts and reduced the expression of inflammatory markers in macrophages. These effects were mediated through the HIF-1ɑ/miR-145-3p and miR-504 pathways. Proteomic analysis revealed that hypoxia-induced changes in the protein expression profile of sEVs, with differentially expressed proteins closely associated with tendon injury repair. Our findings provide compelling evidence that hypoxia-induced sEVs from TSCs play a pivotal role in tendon repair, offering a robust theoretical basis for the development of novel therapeutic strategies targeting tendon injuries.
HostingRepository	iProX
AnnounceDate	2025-06-20
AnnouncementXML	Submission_2025-06-22_23:11:25.998.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang, Wu
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-22 23:11:04	ID requested
⏵ 1	2025-06-22 23:11:26	announced

Dataset with its publication pending

submitter keyword: Tendon Stem Cells, Small Extracellular Vesicles, Hypoxia, HIF-1ɑ, Tendon Healing

Zhaozhu, Li
contact affiliation	The Second Affiliated Hospital of Harbin Medical University
contact email	zhaozhu247@163.com
lab head
Yang, Wu
contact affiliation	The Second Affiliated Hospital of Harbin Medical University
contact email	1095668876@qq.com
dataset submitter

iProX dataset URI