PXD065290

PXD065290 is an original dataset announced via ProteomeXchange.

Title	Evolution of the reproductive interactome and the origins of postmating–prezygotic reproductive divergence
Description	Reproductive proteins evolve rapidly, which is predicted to cause postmating-prezygotic (PMPZ) reproductive isolation between divergent populations. While most previous studies have focused on protein sequence divergence as a likely driver of PMPZ incompatibilities, reproductive proteomes may also diverge compositionally and/or quantitatively. Here, we characterize extensive protein sequence, compositional, and quantitative divergence of male and female reproductive proteomes in two species exhibiting strong PMPZ isolation (D. mojavensis and D. arizonae). We further demonstrate that three divergent male seminal fluid proteins affect the size of the insemination reaction mass and/or fertilization success in D. arizonae. Using quantitative data and protein-protein interaction modeling of proteases and protease inhibitors, we predict the relative importance of protein sequence divergence, compositional changes, and quantitative divergence to the evolution of PMPZ incompatibilities between the species. We find that predicted interspecies protein incompatibilities arise from proteome compositional changes rather than protein sequence divergence, at least for the subset of the interactome that we tested. Furthermore, extensive quantitative divergence, particularly for proteases and inhibitors, suggests pervasive stoichiometric mismatches in heterospecific matings. Altogether, our findings provide novel insights into how the reproductive interactome diverges, and the consequences of this divergence for the evolution of PMPZ reproductive isolation.
HostingRepository	PRIDE
AnnounceDate	2026-04-02
AnnouncementXML	Submission_2026-04-02_09:48:37.015.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD065290
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Luciano Matzkin
SpeciesList	scientific name: Drosophila arizonae; NCBI TaxID: NEWT:7263; scientific name: Drosophila mojavensis mojavensis; NCBI TaxID: NEWT:377586;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-21 15:58:52	ID requested
⏵ 1	2026-04-02 09:48:37	announced

10.6019/PXD065290;

submitter keyword: None

Luciano Matzkin
contact affiliation	Department of Entomology BIO5 Institute Department of Ecology and Evolutionary Biology University of Arizona
contact email	lmatzkin@arizona.edu
lab head
Luciano Matzkin
contact affiliation	University of Arizona
contact email	lmatzkin@arizona.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065290
     1. Label: PRIDE project
     2. Name: Evolution of the reproductive interactome and the origins of postmating–prezygotic reproductive divergence