PXD065280 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Subcellular Proteomic Analyses Reveal REEP5 Knockdown in the Mouse Heart Disrupts Mitochondrial Networks |
| Description | This study investigates the role of REEP5 in maintaining organelle integrity and redox homeostasis in cardiac tissue (specifically cardiomyocytes). Using AAV9-mediated shRNA knockdown in postnatal mice, we acheived a cardiac-specific depletion of REEP5. Hearts were harvested 4 weeks post-injection and subjected to subcellular fractionation to isolate microsomal (SR/ER-enriched), mitochondrial, and cytosolic cellular compartmetns. Each fraction was analyzed using data-independent acquisition (DIA) LC-MS/MS on a Q Exactive Plus instrument and the resulting dataset caputres an organelle-specific proteomic profile following depletion of REEP5. Loss of REEP5 leads to changes in the proteomic profiles of targets involved in regulating SR/ER membrane dynamics, ER-mitochondrial communications, mitochondrial morphology and oxidative stress. This work highlights REEP5 as an important regulator of ER-mitochondrial communication and cardiac homeostasis. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-30 |
| AnnouncementXML | Submission_2026-03-29_19:47:02.674.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Michelle Di Paola |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-20 16:27:04 | ID requested | |
| ⏵ 1 | 2026-03-29 19:47:03 | announced | |
Publication List
| 10.1016/j.mcpro.2026.101527; |
| Di Paola M, Reitz CJ, Kuzmanov U, Jia K, Gramolini AO, Subcellular Proteomic Analyses Reveal REEP5 Knockdown in the Mouse Heart Disrupts Mitochondrial Networks. Mol Cell Proteomics, 25(3):101527(2026) [pubmed] |
Keyword List
Contact List
| Anthony Orlando |
|---|
| contact affiliation | University of Toronto |
| contact email | anthony.gramolini@utoronto.ca |
| lab head | |
| Michelle Di Paola |
|---|
| contact affiliation | Univeristy of Toronto |
| contact email | michelle.dipaola@mail.utoronto.ca |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD065280 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065280
- Label: PRIDE project
- Name: Subcellular Proteomic Analyses Reveal REEP5 Knockdown in the Mouse Heart Disrupts Mitochondrial Networks
