PXD065263

PXD065263 is an original dataset announced via ProteomeXchange.

Title	Creatine kinase B, a downstream effector of MYB, controls migration of osteosarcoma cells via regulation of N-cadherin
Description	A study aimed at identification of proteins associated with CRISPR/Cas-induced depletion of creatine kinase B (CKB) in SAOS-LM5 metastatic osteosarcoma cell line using LC-MS/MS in data-independent acquisition (DIA)-parallel accumulation serial fragmentation (diaPASEF) mode. Here we prepared two KO cell lines, SAOS-LM5 CKB KO 6C and SAOS-LM5 CKB KO 11B, as well as control SAOS-LM5 scrambled cell line. After filter-aided sample preparation (FASP) protein digestion and peptide desalting, the samples were conducted to total proteome analysis. The data acquisition was performed in diaPASEF mode on timsTOF Pro 2 mass spectrometer. Data were processed in Spectronaut 19.0 in directDIA mode. This project covers the raw files from the diaPASEF measurements as well as search results from the Spectronaut 19.0 software.
HostingRepository	PRIDE
AnnounceDate	2026-01-23
AnnouncementXML	Submission_2026-01-23_03:29:14.548.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Bouchal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-20 07:05:59	ID requested
⏵ 1	2026-01-23 03:29:15	announced

Pokludov, á J, Lap, č, í, k P, Zambo IS, Kohoutek J, Zapletalov, á D, M, ú, dry P, Ad, á, mkov, á D, Č, ervinka J, Loja T, Lexa M, Verner J, Š, marda J, Bouchal P, Knopfov, á L, Bene, š P, Creatine kinase B, a downstream effector of c-Myb, controls migration of osteosarcoma cells via regulation of N-cadherin. Cancer Cell Int, 26(1):5(2025) [pubmed]

10.1186/s12935-025-04087-0;

submitter keyword: PASEF, cell migration, creatine kinase B,Data Independent Acquisition, osteosarcoma

Pavel Bouchal
contact affiliation	Masaryk University
contact email	bouchal@chemi.muni.cz
lab head
Pavel Bouchal
contact affiliation	Masaryk University, Faculty of Science
contact email	bouchal@chemi.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD065263

PRIDE project URI

• PRIDE
  1. PXD065263
     1. Label: PRIDE project
     2. Name: Creatine kinase B, a downstream effector of MYB, controls migration of osteosarcoma cells via regulation of N-cadherin