PXD065250

PXD065250 is an original dataset announced via ProteomeXchange.

Title	Comprehensive Proteomic Exploratory of Gingival Crevicular Fluid Identifies Complement and Neutrophil-Associated Alterations in Gingivitis and Periodontitis Progression
Description	Aim: To provide an exploratory proteomic characterization of periodontal disease-related immune pathways by analyzing gingival crevicular fluid, revealing subtle proteomic changes accompanying disease progression. Materials and methods: Gingival crevicular fluid samples were collected from normal (control group), gingivitis, and periodontitis cohorts. Seventy-two samples were investigated, including those from normal (n=12), gingivitis (n=30) and periodontitis (n=30) groups. Samples were analyzed using liquid chromatography–mass spectrometry and sequential statistical analyses including differential expression, weighted gene co-expression network analysis, and protein–protein interaction networks, evaluated with receiver operating characteristic curve analyses. Results: We identified 649 proteins. Differential expression analysis revealed several regulated proteins, including RAC2, S100A12, and LCN2, in the periodontitis group. Weighted gene co‐expression network analysis clustered six protein modules: M1 module was enriched in complement proteins and prominent in gingivitis, whereas neutrophil-related M2 and M5 modules were correlated with periodontitis severity. Protein–protein interaction network analyses revealed hub proteins, including RAC2 and S100A12, highlighting their functional associations. Receiver operating characteristic analysis supported their within-cohort discriminatory potential for selected proteins. Conclusion: This exploratory study suggested the pathophysiological differences in proteome between gingivitis and periodontitis, establishing a foundation for future investigations and hypothesis-driven research.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-04_19:26:52.710.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeong-hun Mok
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-20 01:01:00	ID requested
⏵ 1	2026-01-04 19:26:53	announced

10.1016/j.identj.2025.109306;

Mok JH, Hong JY, Joo M, Yun JH, Park JM, Gingival Crevicular Fluid Proteomic Shift: From Gingivitis to Periodontitis. Int Dent J, 76(1):109306(2026) [pubmed]

submitter keyword: Gingivitis

Periodontitis

Proteomics

Complement

Neutrophils

Jong-Moon Park
contact affiliation	Basilbiotech, Incheon 22002, Republic of Korea.
contact email	basil@basilbiotech.com
lab head
Jeong-hun Mok
contact affiliation	Gachon University Gil Medical Center, Incheon, Republic of Korea
contact email	jeonghunmok@naver.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065250
     1. Label: PRIDE project
     2. Name: Comprehensive Proteomic Exploratory of Gingival Crevicular Fluid Identifies Complement and Neutrophil-Associated Alterations in Gingivitis and Periodontitis Progression