PXD065208

PXD065208 is an original dataset announced via ProteomeXchange.

Title	Proximity Labeling-Based Proteome Analysis of Tax Protein and HBZ RNA using DIA-LC/MS
Description	HTLV-1 is an oncovirus that encodes a transactivator Tax and a latent oncogene HBZ. HTLV-1 early or infectious replication depends on Tax; during HTLV-1 late infection, HBZ plays a crucial role in driving the proliferation of infected cells and maintaining viral persistence. The biphasic replication pattern of HTLV-1 dictated by Tax and HBZ represents a result of viral host adaptation, but how HTLV-1 coordinates Tax and HBZ expression to facilitate early and late infection remains elusive. Here we reveal that HBZ RNA splicing exhibits distinct patterns in Tax+ and Tax- HTLV-1 infected cells. We demonstrate that Tax interacts with the host spliceosome and inhibits HBZ splicing by competitively binding splicing factors including WDR83 and GPATCH1. As a result, Tax confers a natural constraint on HBZ, counterbalancing its anti-replication effect at HTLV-1 early infection, while unleashing HBZ to drive HTLV-1 mitotic propagation during late infection. The splicing-dependent restriction of HBZ by Tax thus represents a critical interplay central to HTLV-1 persistence.
HostingRepository	iProX
AnnounceDate	2025-06-19
AnnouncementXML	Submission_2025-06-19_01:22:17.975.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi Liang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-19 01:22:05	ID requested
⏵ 1	2025-06-19 01:22:18	announced

Dataset with its publication pending

submitter keyword: Tax, HBZ RNA, Proximity Labeling

Guangyong Ma
contact affiliation	China Pharmaceutical University
contact email	gma@cpu.edu.cn
lab head
Yi Liang
contact affiliation	China Pharmaceutical University
contact email	3121070237@stu.cpu.edu.cn
dataset submitter

iProX dataset URI