PXD065159

PXD065159 is an original dataset announced via ProteomeXchange.

Title	Molecular and Physiological Acclimation to Low Light and Iron Scarcity in Globally Abundant Oceanic Pelagophyte
Description	Pelagomonas calceolata is a widely distributed marine alga and is among the most numerous eukaryotes on Earth. It is abundant in subsurface chlorophyll maximum layer (SCML) communities where it can be responsible for a majority of nitrate assimilation. Growth in these communities is frequently limited by the lack of iron (Fe), and no eukaryotic phytoplankton species has been shown to require less Fe than P. calceolata. SCML communities are also light limited, resulting in an increased need for Fe-rich photosynthetic proteins. Consequently, to survive and compete in these SCML environments calls for an Fe/light co-limitation specialist. To understand the strategies behind P. calceolata’s success, we profiled this organism’s physiology and gene expression as it experiences Fe/light co-limitation. Our study describes the cellular changes under steady-state Fe limitation and the short-term responses to Fe resupply. Our culture experiments revealed that P. calceolata maintains exceptionally low Fe:C ratios across conditions and dynamically regulates iron-sparing strategies such as flavodoxin expression and substitution of metal-rich proteins. Furthermore, coupling environmental gene expression with culture-based profiles showed that Fe- and light-responsive genes identified in the lab were strongly enriched in SCML metatranscriptomes, indicating that P. calceolata expresses these adaptations in situ. These results demonstrate low Fe tolerance as a key adaptation enabling P. calceolata to thrive in light-limited marine environments and highlight its broader role in oceanic carbon and nitrogen cycling.
HostingRepository	PRIDE
AnnounceDate	2026-02-24
AnnouncementXML	Submission_2026-02-24_08:13:36.783.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD065159
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Elden Rowland
SpeciesList	scientific name: Pelagomonas calceolata; NCBI TaxID: NEWT:35677;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-18 06:46:52	ID requested
⏵ 1	2026-02-24 08:13:37	announced

10.6019/PXD065159;

submitter keyword: iron and light limitation, proteome,Pelagomonas calceolata

Erin Bertrand
contact affiliation	Associate Professor Biology, Dalhousie University
contact email	erin.bertrand@dal.ca
lab head
Elden Rowland
contact affiliation	Dalhousie University
contact email	erowland@dal.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD065159

PRIDE project URI

• PRIDE
  1. PXD065159
     1. Label: PRIDE project
     2. Name: Molecular and Physiological Acclimation to Low Light and Iron Scarcity in Globally Abundant Oceanic Pelagophyte