PXD065145

PXD065145 is an original dataset announced via ProteomeXchange.

Title	Inegrative Proteomic Analysis Reveals the Cytaskeleton Regulation and Mtophagy Diference Between ischemicCardiomyopathy and Dilated Cardiomyopathy
Description	Ischemic cardiomyopathy (ICM) and dilated cardiomyopathy (DCM) are the two primary etiologies of end-stage heart failure. However, there remains a dearth of comprehensive understanding the global perspective and the dynamics of the proteome and phosphoproteome in ICM and DCM, which hinders the profound comprehension of pivotal biological characteristics as well as differences in signal transduction activation mechanisms between these two major types of heart failure. We conducted highthroughput quantification proteomics and phosphoproteomics analysis of clinical heart tissues with ICM or DCM, which provided us the system-wide molecular insights into pathogenesis of clinical heart failure in both ICM and DCM. Both protein and phosphorylation expression levels exhibit distinct separation between heart failure and normal control heart tissues, highlighting the prominent characteristics of ICM and DCM. By integrating with omics results, Western blots, phosphosite-specific mutation, chemical intervention, and immunofluorescence validation, we found a significant activation of the PRKACA–GSK3β signaling pathway in ICM. This signaling pathway influenced remolding of the microtubule network and regulated the critical actin filaments in cardiac construction. Additionally, DCM exhibited significantly elevated mitochondria energy supply injury compared to ICM, which induced the ROCK1–vimentin signaling pathway activation and promoted mitophagy. Our study not only delineated the major distinguishing features between ICM and DCM but also revealed the crucial discrepancy in the mechanisms between ICM and DCM. This study facilitates a more profound comprehension of pathophysiologic heterogeneity between ICM and DCM and provides a novel perspective to assist in the discovery of potential therapeutic targets for different types of heart failure.
HostingRepository	iProX
AnnounceDate	2025-06-18
AnnouncementXML	Submission_2025-06-18_01:15:16.848.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Linhui Zhai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-18 01:14:54	ID requested
⏵ 1	2025-06-18 01:15:17	announced

Liu M, Zhai L, Yang Z, Li S, Liu T, Chen A, Wang L, Li Y, Li R, Li C, Tan M, Chen Z, Qian J, Integrative Proteomic Analysis Reveals the Cytoskeleton Regulation and Mitophagy Difference Between Ischemic Cardiomyopathy and Dilated Cardiomyopathy. Mol Cell Proteomics, 22(12):100667(2023) [pubmed]

submitter keyword: Ischemic heart disease, Dilated cardiomyopathy, Proteomics, Phosphoproteomics, Pathophysiologic heterogeneity

Minjia Tan
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
contact email	mjtan@simm.ac.cn
lab head
Linhui Zhai
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
contact email	zhailinhui@simm.ac.cn
dataset submitter

iProX dataset URI