PXD065135

PXD065135 is an original dataset announced via ProteomeXchange.

Title	The EGF receptor kinase domain is strongly activated by weak dimerization
Description	The tyrosine kinase domain (TKD) of the epidermal growth factor receptor (EGFR) activates via a unique mechanism of asymmetric dimerization with a partner EGFR TKD rather than a more typical mechanism of activation loop phosphorylation. There is an EGFR oncogenic variant found in patients in which the TKD region of EGFR has been duplicated, resulting in a construct in which two EGFR kinase domain are linked via a natural peptide linker (KDD). KDD is the best approximation of the natural process of EGFR kinase dimerization and activation as well as an example of bona fide oncogenic activation. We studied the structure, enzyme properties, and structural dynamics of KDD to gather insights into the EGFR kinase activation process and its oncogenicity.
HostingRepository	PRIDE
AnnounceDate	2025-11-25
AnnouncementXML	Submission_2025-11-25_06:15:39.115.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zaritza Petrova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-17 19:40:15	ID requested
⏵ 1	2025-11-25 06:15:39	announced

Dataset with its publication pending

submitter keyword: dynamics, KDD, dimerization, kinase, HDX-MS,EGFR

Mark Lemmon
contact affiliation	Yale University, Yale Cancer Biology Institute
contact email	mark.lemmon@yale.edu
lab head
Zaritza Petrova
contact affiliation	Yale University
contact email	zaritza.petrova@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD065135

PRIDE project URI

• PRIDE
  1. PXD065135
     1. Label: PRIDE project
     2. Name: The EGF receptor kinase domain is strongly activated by weak dimerization