PXD065124

PXD065124 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomic Analysis of Paraquat and Alpha Synuclein Neurotoxicity Models in Drosophila
Description	In this project, we sought to perform proteomics in two Drosophila models: neuronally expressed human alpha synuclein model of parkinsonism and a paraquat neurotoxicity model. We exposed wild type Drosophila to 5 mM Paraquat in their food (potato starch flakes) for 7 days post-eclosion (i.e. in adult stage, not larvae). In a separate group, we expressed human alpha synuclein neuronally using the Q-system in the synaptobrevin promoter. Paraquat is a non-selective herbicide used for defoliation of green plant matter prior to harvesting and prolonged exposure has been epidemiologically associated with increased risk of developing Parkinson's Disease (PD). Alpha synuclein is a protein enriched in post-mortem brains of PD patients, primarily in protein aggregates known as Lewy bodies and Lewy neurites that are believed to be central to PD pathophysiology. PD is primarily associated with the progressive loss of dopaminergic neurons in the midbrain, however research has shown that prodromal symptoms (e.g. constipation, lowered gut motility, bladder disturbances) arise years prior to locomotor symptoms. Therefore, we separated the heads and bodies of our paraquat treated and alpha synuclein expressing flies and ran proteomics on each. These data were compared to determine if environmentally induced PD and genetically induced PD share common pathogenic mechanisms and if these changes are more pronounced in the brain or in the body.
HostingRepository	PRIDE
AnnounceDate	2026-09-02
AnnouncementXML	Submission_2026-09-02_10:12:22.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pablo Reina-Gonzalez
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-17 11:31:12	ID requested
⏵ 1	2026-09-02 10:12:22	announced

10.1038/S41531-026-01542-1;

submitter keyword: Heads, Bodies,Drosophila, Paraquat, Proteomics

Souvarish Sarkar
contact affiliation	Envi-Gene Lab, Department of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
contact email	Souvarish_Sarkar@urmc.rochester.edu
lab head
Pablo Reina-Gonzalez
contact affiliation	University of Rochester School of Medicine and Dentistry Department of Environmental Medicine
contact email	pablo_reinagonzalez@urmc.rochester.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD065124

PRIDE project URI

• PRIDE
  1. PXD065124
     1. Label: PRIDE project
     2. Name: Comparative Proteomic Analysis of Paraquat and Alpha Synuclein Neurotoxicity Models in Drosophila