PXD065117 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The deubiquitinase USP17 regulates the expression of the oncogenic driver β-catenin in colorectal cancer |
| Description | Aberrant activation of the Wnt/β-catenin signaling pathway is a hallmark of colorectal cancer (CRC). Here, we identify the deubiquitinating enzyme USP17 as a critical regulator of β-catenin stability in CRC. We demonstrate that USP17 directly interacts with and deubiquitinates β-catenin, preventing its degradation and enhancing its stability. CRISPR/Cas9-mediated knockout of USP17 in CRC-derived cell lines significantly reduced β-catenin levels and suppressed epithelial-mesenchymal transition (EMT), as evidenced by distinct morphological changes and altered expression of classical EMT markers. USP17 depletion reduced the proliferation of CRC cell lines and impaired CRC tumor growth in vivo. Conversely, USP17 overexpression in immortalized rat enterocytes elevated β-catenin levels and enhanced KRAS-induced cell proliferation. RNA sequencing and quantitative proteomic analysis of USP17-depleted CRC cells revealed significant suppression of the transcriptional coactivator function of β-catenin, impacting key oncogenic-related pathways. Our findings establish USP17 as a key regulator of β-catenin signaling and highlight its potential as a candidate therapeutic target in CRC. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-25 |
| AnnouncementXML | Submission_2026-02-25_05:44:51.213.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD065117 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Eric Bonneil |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-17 07:45:34 | ID requested | |
| ⏵ 1 | 2026-02-25 05:44:51 | announced | |
Publication List
Keyword List
| submitter keyword: ubiquitin-proteasome system,USP17, -catenin, colorectal cancer |
Contact List
| Marc Servant |
|---|
| contact affiliation | Université de Montréal |
| contact email | marc.servant@umontreal.ca |
| lab head | |
| Eric Bonneil |
|---|
| contact affiliation | Proteomic Platform |
| contact email | eric.bonneil@umontreal.ca |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD065117 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065117
- Label: PRIDE project
- Name: The deubiquitinase USP17 regulates the expression of the oncogenic driver β-catenin in colorectal cancer
