PXD065110

PXD065110 is an original dataset announced via ProteomeXchange.

Title	A chloroplast protein, PORC, undergoes heat-induced condensation and enhances thermotolerance in Arabidopsis
Description	This project explores the role of chloroplast stress granules (cpSGs), specialized biomolecular condensates that form within chloroplasts under environmental stress, in enhancing plant resilience to heat. Focusing on the chlorophyll biosynthesis enzyme PROTOCHLOROPHYLLIDE OXIDOREDUCTASE C (PORC) in Arabidopsis thaliana, we demonstrate that PORC dynamically localizes to cpSGs in response to both acute (42°C) and prolonged (35°C) heat stress. Although PORC transcription remains heat-insensitive, its protein condenses into discrete puncta inside chloroplasts under elevated temperatures in a reversible and translation-dependent manner. Disruption of PORC function results in impaired thermotolerance, while overexpression enhances photosynthetic recovery, Photosystem II efficiency, and post-stress plant growth. Proteomic analyses of PORC-containing cpSGs reveal enrichment of photosynthetic proteins and stress-responsive factors, indicating that cpSGs function as protective hubs during heat stress. This study uncovers a novel mechanism of chloroplast stress adaptation and suggests that targeting cpSG dynamics and PORC activity could be a promising strategy for developing heat-resilient crops in the face of global climate challenges.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_16:57:38.363.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peng Liu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-17 05:04:23	ID requested
⏵ 1	2026-05-24 16:57:39	announced

10.1093/plphys/kiag220;

Alquraish F, Maruri-L, ó, pez I, Luzarowski M, Hern, á, ndez-S, á, nchez IE, Chodasiewicz M, Chloroplastic protein PORC undergoes heat-induced condensation and enhances thermotolerance in Arabidopsis. Plant Physiol, 201(1):(2026) [pubmed]

submitter keyword: Biomolecular Condensates, Chloroplast, Abiotic Stress,Stress Granules

Monika Chodasiewicz
contact affiliation	Biological and Environmental Science and Engineering Division King Abdullah University of Science and Technology (KAUST)
contact email	monika.chodasiewicz@kaust.edu.sa
lab head
Peng Liu
contact affiliation	KAUST
contact email	peng.liu@kaust.edu.sa
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD065110

PRIDE project URI

• PRIDE
  1. PXD065110
     1. Label: PRIDE project
     2. Name: A chloroplast protein, PORC, undergoes heat-induced condensation and enhances thermotolerance in Arabidopsis