PXD065107

PXD065107 is an original dataset announced via ProteomeXchange.

Title	Evolutionarily conserved E3 ubiquitin ligase modulates carotenoid, sterol and lipid crosstalk
Description	After disrupting the E3 ligase PTR1 in Xanthophyllomyces dendrorhous CBS 6938 (Xd-ptr1), we observed a quadrupling of astaxanthin production, accompanied by increased levels of ergosterol and stellasterol. This disruption also resulted in a reduction of total lipids, a shift in fatty acid composition toward polyunsaturated species, and a decrease in triglyceride levels. To elucidate the mechanism by which PTR1 regulates carotenoid, sterol, and lipid metabolism, we conducted proteomic analyse.
HostingRepository	PRIDE
AnnounceDate	2026-04-09
AnnouncementXML	Submission_2026-04-09_07:08:16.928.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruilin Huang
SpeciesList	scientific name: Phaffia rhodozyma; NCBI TaxID: NEWT:264483;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-17 04:12:19	ID requested
⏵ 1	2026-04-09 07:08:17	announced

10.1073/PNAS.2530496123;

submitter keyword: E3 ubiquitin ligase, Xanthophyllomyces dendrorhous

Shi'an Wang
contact affiliation	1. Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China. 2. Shandong Energy Institute, Qingdao 266101, China. 3. Qingdao New Energy Shandong Laboratory, Qingdao 266101, China.
contact email	wangsa@qibebt.ac.cn
lab head
Ruilin Huang
contact affiliation	Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
contact email	huangrl@qibebt.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD065107

PRIDE project URI

• PRIDE
  1. PXD065107
     1. Label: PRIDE project
     2. Name: Evolutionarily conserved E3 ubiquitin ligase modulates carotenoid, sterol and lipid crosstalk