PXD065102 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Serum H-ferritin to Ferritin ratio as a biomarker of HLH in critically ill patients with hyperferritinemia |
| Description | Ferritin is a sensitive screening parameter to diagnose hemophagocytic lymphohistiocytosis in critically ill patients but lacks specificity. Ferritin is composed of 24 subunits with 2 types of peptide chains: light (FeL) or heavy (FeH). The proportion of H subunit is suggested to increase during hyperinflammation but is not routinely evaluated by the commercial immunoassay. To improve the specificity of the assay for HLH, we performed proteomic analysis to quantify both FeL and FeH in the sera of patients with hyperferritinemia suspected of HLH or with sepsis admitted in intensive care units. Starting from an untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of 6 human serum samples with hyperferritinemia (>5000ug/L), we developed a targeted LC-MS/MS method based on multiple reaction monitoring (MRM) to quantify FeH in human serum and evaluate the diagnostic performance of the L and H subunit (FeL and FeH) in our cohort of critically patients with hyperferritinemia. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-06 |
| AnnouncementXML | Submission_2026-03-05_23:11:37.222.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD065102 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Sara Dufour |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | deamidated residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-17 02:04:18 | ID requested | |
| ⏵ 1 | 2026-03-05 23:11:38 | announced | |
Publication List
| 10.1002/ajh.70189; |
| Debaugnies F, Goetzinger F, Wolff F, Impens F, Dufour S, Van Haver D, Gottignies P, La Schiazza R, Mahadeb B, Meuleman N, Nagant C, Rozen L, Borde P, Corazza F, Serum H-Ferritin-To-Ferritin Ratio as a Biomarker of Hemophagocytic Lymphohistiocytosis in Critically Ill Patients With Hyperferritinemia. Am J Hematol, 101(3):648-653(2026) [pubmed] |
| 10.6019/PXD065102; |
Keyword List
| submitter keyword: LC-MSMS,Human , ferritin, serum |
Contact List
| Debaugnies France |
|---|
| contact affiliation | Laboratory of Translational Research, Centre Hospitalier Universitaire Brugmann, Université libre de Bruxelles, Brussels, Belgium. Medical Biology Department, Laboratoire National de Santé, Dudelange, Luxembourg. Laboratory of Clinical Chemistry, Centre Hospitalier de Luxembourg, Luxembourg |
| contact email | debaugnies.france@chl.lu |
| lab head | |
| Sara Dufour |
|---|
| contact affiliation | VIB-UGent |
| contact email | sara.dufour@vib-ugent.be |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065102
- Label: PRIDE project
- Name: Serum H-ferritin to Ferritin ratio as a biomarker of HLH in critically ill patients with hyperferritinemia
