PXD065094

PXD065094 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	TMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virions
Description	Understanding the relationship between viral proteins and host factors is essential for developing strategies to control virus infections. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells using angiotensin converting enzyme 2 (ACE2) and transmembrane protease, serine 2 (TMPRSS2) as viral receptor and priming protease during the viral entry phase. Here, we report that TMPRSS2 reduces the infectivity of SARS-CoV-2 and HIV-1 during the viral production phase. Treatment of virus-producing cells with a TMPRSS2 inhibitor increases the production of infectious virions. TMPRSS2 enzymatic activity specifically disrupted the trans-Golgi by phosphorylating Golgi stacking proteins through ERK activation, which disturbed virion spike incorporation and structural maturation of the viral envelope glycoproteins, causing lower viral infectivity. We found that the SARS-CoV-2 envelope protein (E protein) counteracts this TMPRSS2 activity to rescue SARS-CoV-2-S incorporation. These results demonstrate negative regulation of viral envelope glycoprotein incorporation by TMPRSS2 and reveal that SARS-CoV-2 regulates the Golgi system to create an optimal viral replication environment.
HostingRepository	jPOST
AnnounceDate	2026-04-15
AnnouncementXML	Submission_2026-05-25_02:27:49.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kazuya Tsumagari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	alpha-amino acetylated residue; S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-16 23:02:02	ID requested
1	2026-04-15 01:10:54	announced
⏵ 2	2026-05-25 02:27:49	announced	2026-05-25: Updated PubMed.

Publication List

Seki S, Harada S, Sugimoto-Ishige A, Unno M, Sakuma M, Ito S, Yamamoto H, Tanabe A, Matsuoka S, Makino-Okamura C, Ki S, Fukuyama H, Harada M, Tsumagari K, Imami K, Saito T, Kubo M, Suzuki T, Koseki H, Matano T, Miyauchi K, TMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virions. EMBO Rep, ():(2026) [pubmed]

Seki S, Harada S, Sugimoto-Ishige A, Unno M, Sakuma M, Ito S, Yamamoto H, Tanabe A, Matsuoka S, Makino-Okamura C, Ki S, Fukuyama H, Harada M, Tsumagari K, Imami K, Saito T, Kubo M, Suzuki T, Koseki H, Matano T, Miyauchi K, TMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virions. EMBO Rep, ():(2026) [pubmed]

Keyword List

submitter keyword: SARS-CoV-2, TMPRSS2, Golgi

submitter keyword: SARS-CoV-2, TMPRSS2, Golgi

Contact List

Kosuke Miyauchi
lab head
Kazuya Tsumagari
contact affiliation	RIKEN
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003877/