PXD065070
PXD065070 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In vivo proximity labeling proteomics with cilia targeted V5-TurboID-eGFP transgenic mouse identified Rpap3 and Ak8 as regulators of motile cilia function |
| Description | Cilia are indispensable for the development and homeostasis of almost all organ systems, and compromised cilia structure and function lead to highly clinical and genetic heterogeneous diseases collected named as ciliopathy. Cilia biology and ciliopathy patho-mechanism remains largely underexplored. So far, cilia research field is lack of a tool that enable identifying cilia proteome in vivo under various physiological and pathological states. We generated cilia-targeted TurboID transgenic mice, and using these mice, we performed data-independent acquisition mass spectrometry (DIA-MS) to identify ciliary polypeptides from streptavidin captured proteomes in the brain and trachea tissues. These mice provide a valuable tool for dissecting ciliary proteomes under various physiological and diseased conditions, which will facilitate the discovering of underlying mechanisms of cilia biology and patho-mechanisms of ciliopathies in multiple organ systems. |
| HostingRepository | iProX |
| AnnounceDate | 2025-06-14 |
| AnnouncementXML | Submission_2025-06-16_19:08:43.834.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Pan Wang |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-06-16 19:08:30 | ID requested | |
| ⏵ 1 | 2025-06-16 19:08:44 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: cilia, proximity labeling (TurboID), transgenic mouse, ciliary proteome, Rpap3, Ak8 |
Contact List
| Ming Ma | |
|---|---|
| contact affiliation | Southwest University |
| contact email | skymaming@swu.edu.cn |
| lab head | |
| Pan Wang | |
| contact affiliation | Southwest University |
| contact email | wangpan940912@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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