PXD065040

PXD065040 is an original dataset announced via ProteomeXchange.

Title	Mammalian mitochondrial polyphosphate is regulated by the 5-InsP7 synthase IP6K1
Description	HEK293T cells were collected and lysed by incubating it in lysis buffer containing 50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 1 mM NaF, 1X protease inhibitor cocktail, 1X phosphatase inhibitor cocktail for 45 mins at 4 degree C followed by centrifugation at 8000g for 10 mins at 4 degree C. Cell lysate containing 1 mg protein was added to 100 microlitre each of 100 micromolar PolyP8-agarose, PolyP3-agarose, Pi agarose, and hydroxy agarose. The volume was made up to 1ml using lysis buffer. The beads were kept overnight at 4 degree C for overnight mixing on an end-over-end mixer, washed using lysis buffer, and boiled at 95 degree C for 5 mins in 1X Laemmli buffer. The proteins were run on 12% SDS-Polyacrylamide gels for 20 mins so that the dye front moves to about 0.8 cm in the resolving gel. The gel was stained using the Coomassie blue stain (0.25%) for half an hour. Later, the gel was destained using a destaining solution to remove excess stain. The stained protein band was cut and sent for Mass spectrometry to TAPLIN facility at Harvard University.
HostingRepository	MassIVE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-16_04:55:39.993.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rashna Bhandari
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-16 03:55:44	ID requested
⏵ 1	2025-06-16 04:55:40	announced

no publication

submitter keyword: Inorganic Polyphosphate, DatasetType:Proteomics

Rashna Bhandari
contact affiliation	Centre for DNA Fingerprinting and Diagnostics
contact email	rashna@cdfd.org.in
lab head
Rashna Bhandari
contact affiliation	Centre for DNA Fingerprinting and Diagnostics
contact email	rashna@cdfd.org.in
dataset submitter

MassIVE dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098211/