PXD065039

PXD065039 is an original dataset announced via ProteomeXchange.

Title	Proteomics data for - Unravelling the mechanism of selective protein hydrolysis by metal-organic frameworks
Description	Metal-organic frameworks (MOFs) are emerging as alternatives to enzymes for selective peptide bond cleavage, which is a crucial procedure in proteomics, protein engineering, and proteolytic drug design. However, the limited understanding behind MOF-assisted protein interactions and hydrolysis mechanisms still severely hinder their development and application. Herein, we explore for the first time the synergy between interactions and reactivity of MOFs towards a whole protein, using both mass spectrometry and molecular docking calculations to understand the cleavage selectivity of UiO-66 towards bovine cytochrome c and hemoglobin and horse myoglobin. Our findings demonstrate that UiO-66 preferentially cleaves peptide bond containing glycine, threonine, and lysine due to the H-bonding and electrostatic interactions between the (positively-charged) “recruiting” residues on the protein surface and linker molecules on the surface of the MOF. This preference is drastically different from the aspartic acid selectivity observed with other Zr-based nanomaterials, and highlights the importance of MOF’s hybrid 3D framework to deliver cleavage specificity in the development of the next generation of MOF-based nanozymes for protein-based fields such as proteomics,biocatalysis and related areas.
HostingRepository	PRIDE
AnnounceDate	2026-04-09
AnnouncementXML	Submission_2026-04-09_07:08:32.011.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD065039
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tamara Vasovic
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-16 02:46:37	ID requested
⏵ 1	2026-04-09 07:08:32	announced

10.1021/ACS.CHEMMATER.6C00428;

10.6019/PXD065039;

submitter keyword: cleavage, hydrolysis, LC-MS, protein,MOFs, UiO-66

prof. dr
contact affiliation	Full professor at Department of Chemistry, Celestijnenlaan 200f - box 2404 3001 Leuven
contact email	tatjana.vogt@kuleuven.be
lab head
Tamara Vasovic
contact affiliation	University of Belgade - Faculty of Chemistry
contact email	tamara.vasovic@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065039
     1. Label: PRIDE project
     2. Name: Proteomics data for - Unravelling the mechanism of selective protein hydrolysis by metal-organic frameworks