⮝ Full datasets listing
PXD065037
PXD065037 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CD46 regulates hepatitis B virus entry by modulating cell-surface NTCP levels through cis-interaction |
| Description | Hepatitis B virus (HBV) infection remains a major global health challenge. While sodium taurocholate co-transporting polypeptide (NTCP) is the primary receptor for HBV entry, the molecular mechanisms regulating NTCP-mediated viral entry remain incompletely understood. Here, we identified CD46 as a crucial regulatory factor for NTCP membrane expression. We found that CD46 interacted with NTCP in cis at the plasma membrane through proximity-based labeling screening. The depletion of CD46 significantly reduced cell-surface NTCP levels and HBV infection in hepatocytes. Anti-CD46 monoclonal antibodies, particularly clone E4.3, inhibited HBV infection by triggering NTCP internalization from the plasma membrane to intracellular vesicles. The antiviral effect of CD46 antibodies was also confirmed in primary human hepatocytes. Our study reveals a previously unknown cis-acting mechanism regulating NTCP-mediated HBV entry and suggests CD46 as a potential therapeutic target for HBV infection. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-01-13 |
| AnnouncementXML | Submission_2026-01-12_20:31:29.104.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yusuke Nakai |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | unknown modification; L-methionine sulfoxide; alpha-amino acetylated residue; S-carboxamidomethyl-L-cysteine |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-06-15 22:07:44 | ID requested | |
| ⏵ 1 | 2026-01-12 20:31:30 | announced |
Publication List
| Miyakawa K, Nakai Y, Kameya T, Nishitsuji H, Watashi K, Takeda M, Seya T, Shimotohno K, Kimura Y, Ryo A, CD46 regulates hepatitis B virus entry by modulating cell-surface NTCP levels through cis-interaction. J Mol Cell Biol, ():(2025) [pubmed] |
Keyword List
| submitter keyword: CD46, Hepatitis B virus |
Contact List
| Kei Miyakawa | |
|---|---|
| lab head | |
| Yusuke Nakai | |
| contact affiliation | Japan Institute for Health Security |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003869/ |
