⮝ Full datasets listing
PXD065015
PXD065015 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | IL17-producing gamma T cells modulate murine tissue fibrosis through stromal cell interactions |
| Description | Tissue injury responses are governed by dynamic immune-stromal crosstalk that can promote productive tissue repair or drive dysregulated extracellular matrix (ECM) production and aberrant vascular remodeling, culminating in fibrosis. Here, we investigate how gamma T cells interact with stromal cells to shape tissue fibrosis using a preclinical model of the biomaterial foreign body response that induces a reproducible, dense fibrotic encapsulation. A mixed population of tissue-resident and migratory gamma delta T cells, consisting of type-1 (gamma delta IFN gamma) and multiple type-17 (gamma delta 17) effector subsets, accumulated at the biomaterial implantation site during the early inflammatory phase of wound healing. While the gamma delta IFN gamma later contracted as tissue fibrosis progressed, the activated gamma delta 17 persisted and served as a dominant source of interleukin (IL)-17. Aging and exposure to a high-fat diet, both factors associated with chronic inflammation and pathological fibrosis, further heightened the gamma delta 17 response. The gamma delta 17 secreted bioactive factors that stimulated fibroblast expression of pro-inflammatory and collagen genes in a Transwell co-culture assay. Computational inference of intercellular communication further linked cytokines and growth factors expressed by gamma delta T cells to the activation of fibroblast and endothelial cell transcription factors involved in ECM remodeling and vascular development, respectively. The elimination of gamma delta T cells with a TCR delta knockout strain resulted in differential expression of ECM and skeletal muscle components as well as altered blood vessel structure within the fibrotic matrix. Altogether, our results propose that signaling from gamma delta T cells can affect stromal cell phenotypes, ultimately influencing the composition and vascularity of fibrotic tissue. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-16 |
| AnnouncementXML | Submission_2026-03-16_09:19:34.538.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Christina King |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-06-13 14:58:37 | ID requested | |
| ⏵ 1 | 2026-03-16 09:19:35 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: fibrosis, DIA-MS, proteomics, muscle, DatasetType:Proteomics |
Contact List
| Birgit Schilling | |
|---|---|
| contact affiliation | Buck Institute |
| contact email | bschilling@buckinstitute.org |
| lab head | |
| Christina King | |
| contact affiliation | Buck Institute for Research on Aging |
| contact email | cking@buckinstitute.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098194/ |
