PXD065010

PXD065010 is an original dataset announced via ProteomeXchange.

Title	Genomic, Proteomic, and Biochemical Study of Pleurotus pulmonarius Secretome and Its Role in Biomass Saccharification
Description	Pleurotus species include edible mushrooms that can grow on a broad range of lignocellulosic substrates by secreting a coordinated system of hydrolytic and oxidative enzymes. This flexibility offers opportunities to valorize agro-industrial residues for production of food and biomaterials using sustainable processes. In this study, we analyzed the genome of Pleurotus pulmonarius LGAM 28684 and investigated its biocatalytic potential for saccharification of pretreated lignocellulose using LC-MS/MS proteomics and biochemical assays. The fungus was cultivated on two lignocellulosic substrates, beechwood and corn stover, as well as xylose as a control. The fungus was cultivated on beechwood, corn stover, and xylose. Beechwood induced the richest secretome with abundant oxidases. The corn stover secretome had fewer proteins but was focused on carbohydrate-acting enzymes, with abundant polysaccharide-degrading and accessory enzymes. Despite lower enzyme diversity, the corn stover secretome achieved higher lignocellulose saccharification, further improved by oxidoreductase inhibition. Supplementing an industrial cellulase cocktail with P. pulmonarius corn stover secretomes enhanced sugar release by 40%. These findings highlight the dynamic enzymatic response of P. pulmonarius to lignocellulosic substrates and its potential in biomass valorization and the design of enzymatic cocktails.
HostingRepository	PRIDE
AnnounceDate	2025-11-03
AnnouncementXML	Submission_2025-11-03_02:56:09.337.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD065010
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Romanos Siaperas
SpeciesList	scientific name: Pleurotus pulmonarius; NCBI TaxID: NEWT:28995;
ModificationList	acetylated residue; deamidated L-asparagine; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-13 11:52:18	ID requested
⏵ 1	2025-11-03 02:56:10	announced

10.1021/acs.jafc.5c09170;

Siaperas R, Papadaki E, Giannikos P, Zerva A, Topakas E, Secretome and Its Role in Biomass Saccharification. J Agric Food Chem, 73(44):28539-28551(2025) [pubmed]

10.6019/PXD065010;

submitter keyword: None

Dr Evangelos
contact affiliation	Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
contact email	vtopakas@chemeng.ntua.gr
lab head
Romanos Siaperas
contact affiliation	Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
contact email	roman_siap@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD065010

PRIDE project URI

• PRIDE
  1. PXD065010
     1. Label: PRIDE project
     2. Name: Genomic, Proteomic, and Biochemical Study of Pleurotus pulmonarius Secretome and Its Role in Biomass Saccharification