PXD064950

PXD064950 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Analysis of Mouse Plasma using Tandem-Mass Tagging (TMT)
Description	The risk of breast cancer increases decades after ionizing radiation exposure, thereby linking aging intrinsically to the evolution of cancer. We hypothesized that radiation accelerates aging and carcinogenesis through similar pathways, specifically low-grade systemic inflammation. Here, we used the radiation-genetic mammary chimera model to examine the differential expression of 532 plasma proteins in BALB/c female mice between radiation exposure and experiment termination at 18 months. Mice were sham-irradiated or irradiated with 50 cGy prior to being orthotopically transplanted with syngeneic Trp53 null mammary epithelium and half were treated for 6 months with anti-inflammatory low-dose aspirin. Plasma was collected at 4, 8, and 18 months from non-tumor-bearing mice and from those that had developed tumors between 12 and 18 months. Plasma quantitative proteomic analysis identified significant alterations in proteins involved in the inflammatory response in irradiated mice as a function of age. Levels of C4b-binding protein were decreased at 4 months in irradiated mice compared to controls, which was blocked in aspirin-treated irradiated mice. Notable differences in the expression of proteins associated with the inflammation were evident in tumor-bearing versus similarly aged mice. Complement components C1qA, C1qB, and C1qC were significantly increased in tumor-bearing mice that had been irradiated, whereas similarly aged mice without tumors displayed a decline in complement system activity. The specific changes in the complement system, which mediates adaptive immune function, following radiation exposure may contribute to cancer progression as a function of age.
HostingRepository	PRIDE
AnnounceDate	2025-07-16
AnnouncementXML	Submission_2025-07-15_17:21:48.749.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064950
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jingyun Lee
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-12 11:36:02	ID requested
⏵ 1	2025-07-15 17:21:49	announced

10.6019/PXD064950;

submitter keyword: mouse, TMT, plasma, bottom-up proteomics, LC-MS/MS

Mary Helen Barcellos-Hoff
contact affiliation	Department of Radiation Oncology and Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 2340 Sutter Street, San Francisco, CA, USA
contact email	Maryhelen.barcellos-hoff@ucsf.edu
lab head
Jingyun Lee
contact affiliation	Wake Forest University School of Medicine
contact email	jilee@wakehealth.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064950
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Analysis of Mouse Plasma using Tandem-Mass Tagging (TMT)