PXD064908

PXD064908 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Label Free Quantitative Proteomic Analysis of Age-Related Protein Expression Changes in Mouse Brains
Description	For this analysis, four young (2-month-old) and four aged (24-month-old) mice were used. The mouse brains were quickly dissected and sampled according to the procedure below. The proteomic analysis of PTS-soluble proteins was performed as follows: Homogenization and Collection of the Soluble Fraction: Mouse brain tissue is pulverized and then homogenized in PTS (Phase Transfer Surfactant) buffer. The homogenate is subsequently centrifuged at 15,000 × g for 15 minutes, and the resulting supernatant is collected as the "PTS-soluble fraction". Protein Reduction, Alkylation, and Digestion: The proteins within the collected supernatant (30 µg) are first reduced with DTT (dithiothreitol), followed by an alkylation step. The proteins are then digested with the enzyme trypsin to produce peptide fragments suitable for analysis. Peptide Purification: After the digested sample is treated with trifluoroacetic acid (TFA), the peptides are purified using a GL-tip SDB solid-phase extraction column. Mass Spectrometry: The purified peptides are subjected to label-free quantitative proteomic analysis using a Q-Exactive mass spectrometer.
HostingRepository	jPOST
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_08:00:19.539.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064908
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	SHirayama
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	L-cysteine methyl disulfide; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-11 20:26:46	ID requested
⏵ 1	2025-08-03 08:00:19	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Aging, Protein aggregation

submitter keyword: Aging, Protein aggregation

Contact List

Shigeo Murata
lab head
SHirayama
contact affiliation	Hamamatsu University School of Medicine
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003863/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003863/