PXD064900

PXD064900 is an original dataset announced via ProteomeXchange.

Title	Dataset accompanying "Influences of Gaseous Substrate Composition on Single Cell Protein and Poly-3-hydroxybutyrate Production by Cupriavidus necator"
Description	Abstract. Microbial gas fermentation remains a promising biotechnology for the production of a variety of industrially relevant chemicals under relatively benign conditions using ubiquitous feedstocks, such as carbon dioxide. Implementation of these gas fermentation processes requires an understanding of the microbial response to different ratios of the gaseous feedstocks (carbon dioxide, oxygen, and hydrogen) supplied to the reactor. Here, we used Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) as a microbial biocatalyst due to its metabolic diversity, genetic tractability, and ability to grow to high cell densities during aerobic fermentation in bioreactors. Specifically, we investigate whether the supply of different proportions of hydrogen and carbon dioxide in the gas stream (1:1, 3:1, and 8:1 H2:CO2) affects the physiology of C. necator, with a focus on the accumulation of single cell protein (SCP) and poly-3-hydroxybutyrate (PHB) within the microbial biomass. We found that an 8:1 ratio of H2:CO2 led to both the highest overall biomass production and PHB content within the biomass after a seven-day incubation. Intracellular SCP content varied as a function of both length of incubation and gas ratio supplied. We conducted a proteomic analysis to determine whether differences in productivity could be correlated with changes in protein expression over time and across treatment groups. This study highlights the importance of considering gaseous substrate composition and its potential effects on microbial physiology and metabolism during fermentation.
HostingRepository	PRIDE
AnnounceDate	2026-05-16
AnnouncementXML	Submission_2026-05-16_06:58:17.590.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD064900
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	J Hervey
SpeciesList	scientific name: Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha); NCBI TaxID: NEWT:381666; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-11 18:47:28	ID requested
⏵ 1	2026-05-16 06:58:18	announced

10.6019/PXD064900;

submitter keyword: None

J Hervey
contact affiliation	Center for Bio/Molecular Science & Engineering, NRL-DC
contact email	judson.hervey@nrl.navy.mil
lab head
J Hervey
contact affiliation	NRL
contact email	william.j.hervey.civ@us.navy.mil
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD064900

PRIDE project URI

• PRIDE
  1. PXD064900
     1. Label: PRIDE project
     2. Name: Dataset accompanying "Influences of Gaseous Substrate Composition on Single Cell Protein and Poly-3-hydroxybutyrate Production by Cupriavidus necator"