PXD064897

PXD064897 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Co-Immunoprecipitation of TMEM65
Description	Mitochondrial Ca2+ transport is a critical process in cellular physiology. One pathway for export occurs via Na+/Ca2+exchange (mito-NCX) machinery. TMEM65 has recently been shown to be crucial for mito-NCX activity, but its precise mechanistic role remains unknown. To evaluate whether TMEM65 forms a complex with NCLX, a protein previously proposed to facilitate mitochondrial Na+/Ca2+ exchange transport, and to gain insights into TMEM65’s potential functions, we sought to characterize potential interactors of TMEM65, performing co-immunoprecipitation on TMEM65 containing a C-terminal ID4 tag.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-08_16:08:58.705.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shouling Xu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-11 15:57:29	ID requested
⏵ 1	2026-02-08 16:08:59	announced

Publication List

Zhang JL, Chang YC, Lai PH, Yeh HI, Tsai CW, Huang YL, Liu TY, Lee IC, Foulon N, Xu Y, Rao B, Shih HM, Tu YC, Reyes AV, Xu SL, Feng L, Tsai MF, exchanger. Nat Cell Biol, 27(8):1301-1310(2025) [pubmed]
10.1038/s41556-025-01721-x;

Zhang JL, Chang YC, Lai PH, Yeh HI, Tsai CW, Huang YL, Liu TY, Lee IC, Foulon N, Xu Y, Rao B, Shih HM, Tu YC, Reyes AV, Xu SL, Feng L, Tsai MF, exchanger. Nat Cell Biol, 27(8):1301-1310(2025) [pubmed]

10.1038/s41556-025-01721-x;

Keyword List

submitter keyword: Co-IP, LC-MS/MS

submitter keyword: Co-IP, LC-MS/MS

Contact List

Shou-Ling Xu
contact affiliation	Department of Plant Biology, Carnegie Institution for Science, Stanford, California, USA
contact email	sxu@carnegiescience.edu
lab head
Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064897
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064897

PRIDE project URI

Repository Record List

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