PXD064871

PXD064871 is an original dataset announced via ProteomeXchange.

Title	Deep Coverage Multi-omic Profiling of C2C12 Dexamethasone Skeletal Muscle Atrophy
Description	Skeletal muscle atrophy and weakness are major contributors to morbidity, prolonged recovery, and long-term disability across a wide range of diseases. Atrophy is caused by breakdown of sarcomeric proteins resulting in loss of muscle mass and strength. Molecular mechanism underlying the onset of muscle atrophy and its progression have been analysed in patients, mice, and cell culture but the complementarity of these model systems remains to be explored. Here, we applied deep-coverage transcriptomic and proteomic profiling to characterize dynamic changes during dexamethasone-induced atrophy in the widely used murine skeletal muscle cell line C2C12. Comparison with published datasets confirmed that muscle differentiation is well recapitulated in C2C12 myotubes. Under dexamethasone-induced treatment, this model was particularly suited to capture early atrophy events, prior to disassembly and degradation of sarcomeric proteins. We also identified alterations in mitochondrial gene expression and differential alternative splicing events during early-stage myotube atrophy. This dataset complements existing in vivo data and provides novel insights into the regulatory processes during skeletal muscle wasting.
HostingRepository	PRIDE
AnnounceDate	2025-10-14
AnnouncementXML	Submission_2025-10-14_06:27:40.273.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Popp
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-11 03:53:41	ID requested
⏵ 1	2025-10-14 06:27:40	announced

10.1152/AJPCELL.00518.2025;

submitter keyword: Differentiation,C2C12, Skeletal muscle atrophy, Dexamethasone, TMT-MS

Suzuka Nakagawa
contact affiliation	Max-Delbrück Center for Molecular Medicine, Robert-Rössle Str. 10, 13125 Berlin, Germany
contact email	Suzuka.Nakagawa@mdc-berlin.de
lab head
Oliver Popp
contact affiliation	MDC Berlin
contact email	oliver.popp@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD064871

PRIDE project URI

• PRIDE
  1. PXD064871
     1. Label: PRIDE project
     2. Name: Deep Coverage Multi-omic Profiling of C2C12 Dexamethasone Skeletal Muscle Atrophy