PXD064869

PXD064869 is an original dataset announced via ProteomeXchange.

Title	miR-423-5p target identification with proteomics in HCC
Description	Hepatocellular carcinoma (HCC) remains a significant clinical challenge due to limited diagnostic and therapeutic options. Non-coding RNAs, such as microRNAs (miRNAs), play key roles in cancer biology. Our previous findings showed that miR-423-5p exerts anti-cancer effects on HCC patients treated with sorafenib by promoting autophagy. In this study, we investigated the molecular mechanisms underlying its activity by generating SNU-387 HCC cell line stably overexpressing miR-423-5p and conducting a comprehensive proteomic analysis. Mass spectrometry profiling identified 698 differentially expressed proteins (DEPs) in miR-423-5p-overexpressing cells compared to controls. Functional enrichment analysis revealed significant alterations in metabolic pathways, particularly purine/pyrimidine metabolism and gluconeogenesis. To relate these findings to clinical context, we integrated experimentally validated and predicted miR-423-5p targets with The Cancer Genome Atlas (TCGA) Liver Hepatocellular Carcinoma (LIHC) dataset. Seven candidate proteins were significantly associated with patient prognosis (log-rank p < 0.05 for both overall and disease-free survival). These targets were downregulated in our miR-423-5p model but found to be upregulated in stage III HCC tissues from TCGA data.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:25:01.079.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Boocock
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-11 01:59:09	ID requested
⏵ 1	2025-09-28 16:25:01	announced

Luce A, Bocchetti M, Cossu AM, Tathode MS, Boocock DJ, Coveney C, Romano MP, De Iesu MR, Simeone I, Mele L, Vitale G, Sperlongano R, Misso G, Verderio EAM, Zappavigna S, Caraglia M, Proteomic profiling identifies miR-423-5p as a modulator of oncogenic metabolism in HCC. J Transl Med, 23(1):1008(2025) [pubmed]

10.1186/s12967-025-07039-4;

submitter keyword: miR-423-5p, tripletof 6600, Hepatocellular carcinoma, human,HCC

David Boocock
contact affiliation	John van Geest Cancer Research Centre, Nottingham Trent University, UK
contact email	david.boocock@ntu.ac.uk
lab head
David Boocock
contact affiliation	Nottingham Trent University
contact email	david.boocock@ntu.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD064869

PRIDE project URI

• PRIDE
  1. PXD064869
     1. Label: PRIDE project
     2. Name: miR-423-5p target identification with proteomics in HCC